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Updated: Aug 11, 2025

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

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Probing the human epidermis by combining ToF-SIMS and multivariate analysis.

Xavier Delvaux¹, Céline Noël², Yves Poumay³

¹Laboratoire Interdisciplinaire de Spectroscopie Electronique (LISE), Namur Institute of Structured Matter (NISM), Namur 5000, Belgium.

|February 8, 2023

Summary

Researchers developed a new method using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) to analyze the human epidermis. This technique successfully distinguishes between different skin cell layers, aiding in understanding skin barrier function and disease.

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Published on: September 26, 2019

Area of Science:

Biomedical Engineering
Materials Science
Dermatology

Background:

The mammalian epidermis forms a crucial barrier against environmental threats.
Skin pathologies can disrupt epidermal barrier function, often with complex molecular underpinnings.
Understanding molecular changes in the epidermis is vital for treating skin diseases.

Purpose of the Study:

To develop a reproducible methodology for characterizing the human epidermis using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS).
To apply ToF-SIMS to an in vitro model of the human epidermis, specifically reconstructed human epidermis (RHE).
To extract meaningful biological information and distinguish epidermal layers using multivariate analysis.

Main Methods:

Static ToF-SIMS characterization of RHE cryosections.
High mass and high lateral resolution acquisitions.
Principal Components Analysis (PCA) for multivariate data analysis.

Main Results:

Successful distinction between cornified layers and metabolically active epidermal cells using PCA on ToF-SIMS data.
Demonstration of ToF-SIMS's capability to extract biologically relevant spectral features.
Validation of the methodology for producing experimental replicates in RHE models.

Conclusions:

ToF-SIMS, combined with PCA, provides a powerful and reproducible method for characterizing the human epidermis at a molecular level.
This approach enhances the study of skin barrier function and the molecular basis of skin pathologies.
The methodology facilitates reliable ex vivo analysis of epidermal models, aiding in research and therapeutic development.