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In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
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SNHG1 functions as a ceRNA in hypertrophic scar fibroblast proliferation and apoptosis through miR-320b/CTNNB1 axis

Qiaoling Li1, Bowei Zhang2, Jie Lu3

  • 1Center of Medical Cosmetology, Chengdu Second People's Hospital, No. 10, Qingyun South Street, Jinjiang District, Chengdu, 610011, Sichuan, China. liqiaoling1005@126.com.

Archives of Dermatological Research
|February 8, 2023
PubMed

Insights

Small nucleolar RNA host gene 1 (SNHG1) promotes hypertrophic scar fibroblast growth by upregulating catenin beta 1 (CTNNB1) via the microRNA-320b (miR-320b) pathway. This ceRNA network impacts fibroblast proliferation and apoptosis.

Area of Science:

  • Fibrotic disease research
  • Molecular biology
  • RNA regulation

Background:

  • Hypertrophic scarring (HS) is a fibrotic skin condition.
  • Competing endogenous RNA (ceRNA) networks regulate cell phenotypes.
  • The role of catenin beta 1 (CTNNB1) in HS requires elucidation.

Purpose of the Study:

  • Investigate CTNNB1's effect on hypertrophic scar fibroblasts (HSFBs).
  • Determine CTNNB1's function within a ceRNA network.
  • Elucidate the SNHG1/miR-320b/CTNNB1 axis in HS.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (RT-qPCR) for RNA expression.
  • Cell Counting Kit-8 (CCK-8) assay and flow cytometry for proliferation and apoptosis.
  • RNA pull down, luciferase reporter, and RNA-binding protein immunoprecipitation (RIP) assays for mechanism analysis.

Main Results:

  • CTNNB1 expression was elevated in HSFBs.
  • CTNNB1 depletion inhibited HSFB proliferation and promoted apoptosis.
  • SNHG1 acted as a ceRNA, upregulating CTNNB1 by sponging miR-320b.
  • CTNNB1 overexpression reversed SNHG1 depletion effects.

Conclusions:

  • SNHG1 promotes HS by regulating HSFB proliferation and apoptosis via the miR-320b/CTNNB1 axis.
  • The SNHG1/miR-320b/CTNNB1 ceRNA network is a key player in hypertrophic scar formation.
  • Targeting this pathway may offer therapeutic strategies for hypertrophic scars.
Keywords:
CTNNB1Hypertrophic scar fibroblastProliferationSNHG1miR-320b

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