Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

1.3K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
1.3K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

1.1K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.1K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

2.0K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
2.0K
Secondary Lymphoid Organs01:15

Secondary Lymphoid Organs

1.8K
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
1.8K
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

11.6K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
11.6K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

7.6K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between cumulative glucocorticoid exposure and epicardial adipose tissue volume in systemic lupus erythematosus.

EULAR rheumatology open·2026
Same author

Asymmetrical muscular disorder with normal creatine kinase (CK) and electromyography (EMG): the challenging diagnosis of muscular polyarteritis nodosa (MPAN), case report and literature review.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

Glomeruloid haemangioma in Erdheim-Chester disease: An atypical skin manifestation associated with elevated vascular endothelial growth factor-A levels.

British journal of haematology·2026
Same author

Xanthogranulomatous Inflammatory Pelvic Mass Mimicking Malignancy: Successful Conservative Treatment and Narrative Insights into Diagnosis and Management.

Journal of clinical medicine·2026
Same author

Clonal expansion of CAR-T cells harboring lentivector integration in the PLAAT4 gene following anti-CD19 CAR T-cell therapy in a context of cutaneous T-cell lymphoma.

Haematologica·2026
Same author

Deep Learning Predicts Mutations and Outcomes in Gastrointestinal Stromal Tumors from Whole-Slide Images.

Cancer research·2026
Same journal

Adult sialoblastoma harbouring FGFR2 p.C382R gene mutation.

Virchows Archiv : an international journal of pathology·2026
Same journal

Response to: comment on "FOXL2 expression in dysgenetic gonads supports the diagnostic possibility of ovotesticular differences of sex development".

Virchows Archiv : an international journal of pathology·2026
Same journal

Role of INSM1 expression in the diagnostic work-up of pancreatic acinar cell carcinoma with special reference to the differential diagnosis with neuroendocrine tumor.

Virchows Archiv : an international journal of pathology·2026
Same journal

Mismatch repair protein deficiency (MMRd) and high microsatellite instability (MSI-H) in gastrointestinal stromal tumour (GIST) - a case report and review of the literature of this exceedingly rare phenotype.

Virchows Archiv : an international journal of pathology·2026
Same journal

Primary renal synovial sarcoma: a clinicopathological study of 23 cases highlighting the diagnostic value of SS18-SSX, TLE1, and INI1 immunohistochemistry panel.

Virchows Archiv : an international journal of pathology·2026
Same journal

Clinicopathologic characteristics of uterine malignant epithelioid neoplasms with EWSR1::CREM fusion: Report of 2 cases at a novel site with literature review.

Virchows Archiv : an international journal of pathology·2026
See all related articles

Related Experiment Video

Updated: Aug 11, 2025

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.6K

OCT2 expression in histiocytoses.

Irena Antonia Ungureanu1, Fleur Cohen-Aubart2, Sébastien Héritier3

  • 1Pathology Department, Paris-Saclay University, Versailles SQY University (UVSQ), EA4340-BECCOH, Assistance Publique-Hôpitaux de Paris (APHP), Ambroise-Paré Hospital, 9 Avenue Charles de Gaulle, 92104, Boulogne-Billancourt, France.

Virchows Archiv : an International Journal of Pathology
|February 8, 2023
PubMed
Summary
This summary is machine-generated.

OCT2 nuclear expression aids in diagnosing histiocytosis, showing 100% sensitivity for Rosai-Dorfman disease (RDD) and its variants. This marker is also valuable for H syndrome-related histiocytoses but less specific for Erdheim-Chester disease.

Keywords:
H syndromeHistiocytosisMixed histiocytosisOCT2Rosai-Dorfman disease

More Related Videos

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
07:43

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Published on: July 29, 2017

9.7K
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K

Related Experiment Videos

Last Updated: Aug 11, 2025

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

11.6K
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
07:43

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Published on: July 29, 2017

9.7K
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K

Area of Science:

  • Pathology
  • Immunohistochemistry
  • Histiocytic Disorders

Background:

  • Distinguishing reactive from neoplastic histiocytes is a diagnostic challenge in histiocytosis.
  • Previous research indicated OCT2 nuclear expression in Rosai-Dorfman disease (RDD).

Purpose of the Study:

  • To evaluate OCT2 nuclear expression across a wider spectrum of sporadic and H syndrome-related histiocytoses.
  • To determine the utility of OCT2 as a diagnostic marker for various histiocytic disorders.

Main Methods:

  • Retrieved 156 histiocytosis samples from Ambroise Paré Pathology Department.
  • Reviewed all slides and molecular analyses.
  • Performed OCT2 immunohistochemical staining on all samples.

Main Results:

  • OCT2 was 100% sensitive for RDD and mixed histiocytoses with an RDD component.
  • OCT2 was negative in 91% of Erdheim-Chester disease (ECD) cases.
  • OCT2 expression was observed in 41% of Langerhans cell histiocytosis (LCH) and 38% of juvenile xanthogranuloma (JXG) cases.
  • All H syndrome-associated histiocytoses (n=10) were OCT2 positive, regardless of histology.
  • OCT2 was negative in granulomatous lymphadenitis.

Conclusions:

  • OCT2 is a highly sensitive marker for RDD and its variants.
  • OCT2 expression is variable in LCH and JXG but largely absent in ECD.
  • OCT2 is a useful pan-histiocytic marker for H syndrome-related disorders.