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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Two interacting basic helix-loop-helix transcription factors control flowering time in rice.
Yanbin Yin1, Zhiqiang Yan1, Jianing Guan1
1Rice Research Institute, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang 110866, China.
Plant Physiology
|February 9, 2023
Summary
Two rice transcription factors, Hd1 Binding Protein 1 (HBP1) and Partner of HBP1 (POH1), regulate flowering time by controlling the Heading date 1 (Hd1) gene. Their actions impact rice adaptation and yield.
Area of Science:
- Plant Molecular Biology
- Genetics
- Agronomy
Background:
- Flowering time is crucial for rice adaptation and yield.
- Heading date 1 (Hd1) is a key regulator of photoperiodic control in rice flowering.
Purpose of the Study:
- To identify and characterize transcriptional regulators of Hd1.
- To elucidate the roles of HBP1 and POH1 in rice flowering time control.
Main Methods:
- Generation of HBP1 knockout mutants and transgenic lines overexpressing HBP1 and POH1.
- Analysis of gene expression and protein interactions.
- CRISPR/Cas9 gene editing.
Main Results:
- HBP1 and POH1 form dimers and directly bind to the Hd1 promoter to activate its expression.
- HBP1 knockout accelerated flowering; overexpression of HBP1 and POH1 delayed flowering.
- Overexpression suppressed key flowering genes like Ehd1, Hd3a, and RFT1.
Conclusions:
- HBP1 and POH1 act as transcriptional regulators of Hd1, controlling rice flowering time.
- This provides a mechanism for modulating rice growth period, adaptation, and yield.
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