Three new pentacyclic triterpenes isolated from Strychnos henningsii Gilg. and their cytotoxic evaluation

Three new pentacyclic triterpenes, henninglupal (3), 19-ethylene henningsyl (4), and 19-ethylene henningsal (6), along with the previously reported crystal heninngsal (2) and alpha-amyrin acetate (5) were isolated from a methanolic extract of the bark of Strychnos henningsii Gilg. The new pentacyclic triterpenes were elucidated through comprehensive spectroscopic analysis, including 1D, 2D NMR, and High-resolution LCMS. The in vitro MTT cytotoxicity of compound 2 - 6 was evaluated on Caco-2 cell lines, where all the isolated compounds exhibited low cytotoxic effects up to concentrations of 125 µM.