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Quantification of kininogen in human renal medulla
J Hallbach1, G Adams, G Wirthensohn
1Institut für Klinische Chemie, Städt, Krankenhaus München-Bogenhausen.
Biological Chemistry Hoppe-Seyler
|September 1, 1987
Summary
Researchers found renal kininogen in human kidney tissue and tubules. This study identifies a novel medullary kininase activity and its inhibition, contributing to understanding kidney kinin system regulation.
Area of Science:
- Nephrology
- Biochemistry
- Physiology
Background:
- The kinin-kallikrein system plays a role in kidney function.
- Renal kininogen localization and metabolism are not fully understood.
Purpose of the Study:
- To investigate the presence and localization of renal kininogen.
- To characterize kinin release and degradation in the human kidney medulla.
Main Methods:
- Detection of renal kininogen in medullary tissue and tubule suspensions.
- Measurement of liberated kinin using bradykinin radioimmunoassay.
- Assessment of kinin degradation by endogenous kidney enzymes.
Main Results:
- Renal kininogen was identified in human medullary tissue and tubules.
- A novel endogenous medullary kininase activity was observed, distinct from kininase I and II.
- This medullary kininase was inhibited by heat or mercuric chloride, with kinin release of 9-26 nmol/g protein.
- Renal cortical kininogenase activity was inhibited by aprotinin.
Conclusions:
- Renal kininogen is present in human kidney medulla and tubules.
- A unique medullary kininase degrades kinins, suggesting localized regulation of the kinin system.
- These findings provide insights into the intrarenal kinin system's physiology.