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Related Concept Videos

CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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Related Experiment Video

Updated: Aug 11, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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[CRISPR/Cas9: From research to therapeutic application].

T Ben Yacoub1, J Wohlschlegel1, J-A Sahel2

  • 1Sorbonne université, Inserm, CNRS, institut de la Vision, 75012 Paris, France.

Journal Francais D'Ophtalmologie
|February 9, 2023
PubMed
Summary

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) is an advanced genome editing tool superior to ZFN and TALEN. This technology offers new hope for treating genetic disorders like cancer and improving disease modeling.

Keywords:
CRISPR/Cas9Gene therapyGenome engineeringIngénierie du génomeThérapie génique

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • Genome engineering is crucial for understanding and treating genetic disorders.
  • CRISPR/Cas9, zinc-finger nucleases (ZFN), and transcription activator-like effectors (TALEN) are key genome editing technologies.
  • CRISPR/Cas9 has emerged as the most efficient tool compared to its predecessors.

Purpose:

  • To highlight the efficiency and applications of CRISPR/Cas9 technology in genome editing.
  • To discuss the role of CRISPR/Cas9 in developing treatments for diseases and in genetic disease modeling.
  • To acknowledge the ongoing research and potential of CRISPR/Cas9 in therapeutic development.

Summary:

  • CRISPR/Cas9, a bacterial adaptive immune system, enables precise gene cutting and modification across diverse organisms.
  • It surpasses ZFN and TALEN in efficiency for genome editing applications.
  • The technology is being actively developed for treating cancers, cardiovascular, and ophthalmic disorders, and for creating accurate genetic disease models.

Impact:

  • CRISPR/Cas9 offers significant advancements in therapeutic research for genetic diseases.
  • It provides researchers with powerful tools for genetic disease modeling and understanding pathological mechanisms.
  • Despite ethical considerations, CRISPR/Cas9 represents a hopeful frontier in global health innovation.