Circulating miR-340-5p and miR-506-3p as Two Osteo-miRNAs for Predicting Osteoporosis in a Cohort of Postmenopausal Women
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Published on: August 2, 2024
Zifeng Lu1, Haiou Cao2, Xiaoyin Hu3
1Department of Orthopedics (No. 1), Heilongjiang Beidahuang Group General Hospital, Harbin, Heilongjiang 150088, China.
Objective:
An increasing risk of developing osteoporosis which is characterized by bone production weakness and microarchitectural deterioration is found among postmenopausal women. MicroRNAs (miRNAs) are secreted into the circulation from cells of various tissues in response to local disease severity including bone diseases. Herein, we set out to identify candidate miRNAs predictable for osteoporosis incidence in postmenopausal elderly women.
Methods:
The circulating miRNA expression profiles deposited in the dataset accessioned as GSE201543 were downloaded from the GEO database. The study included 176 postmenopausal women who underwent BMD testing, including 96 women reporting osteoporosis and 70 women reporting normal BMD. All subjects were submitted their serum samples for measurements of bone metabolism markers.
Results:
The miRNA expression profiles of the GSE201543 dataset were differentially analyzed and found 97 miRNAs being upregulated concomitantly with 31 miRNAs being downregulated in the serum samples between osteoporotic postmenopausal women and postmenopausal women with normal BMD. Osteoporotic postmenopausal women were demonstrated with elevated serum levels of miR-340-5p and miR-506-3p when compared to normal postmenopausal women. Pearson correlation analysis demonstrated that circulating miR-340-5p and miR-506-3p expressions were increased as BAP, β-CTx, and PINP levels increased, but osteocalcin and 25-(OH)VitD levels are declined in osteoporotic postmenopausal women. Results of the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) showed circulating miR-340-5p and miR-506-3p expressions alone or combined together produced 0.843 AUC, 0.851 AUC, and 0.935 AUC, respectively, when used to predict the incidence of osteoporosis in postmenopausal women.
Conclusion:
Our work suggested that circulating miR-340-5p and miR-506-3p function as osteo-miRNAs in postmenopausal women and may serve as potential noninvasive biomarkers for the incidence of osteoporosis in postmenopausal women.
Insights
Circulating microRNAs (miRNAs) miR-340-5p and miR-506-3p show promise as noninvasive biomarkers for predicting osteoporosis in postmenopausal women. These osteo-miRNAs may aid in early detection and management of bone density loss.
Area of Science:
- Biochemistry
- Genetics
- Gerontology
Background:
- Postmenopausal osteoporosis is a growing concern, characterized by reduced bone strength and deterioration.
- Circulating microRNAs (miRNAs) are released from tissues and reflect local disease states, including bone health.
Purpose of the Study:
- To identify specific circulating miRNAs that can predict the incidence of osteoporosis in postmenopausal women.
- To investigate the potential of these miRNAs as noninvasive biomarkers for osteoporosis.
Main Methods:
- Downloaded and analyzed circulating miRNA expression profiles from the GEO dataset GSE201543.
- Included 176 postmenopausal women (96 with osteoporosis, 70 with normal BMD) and measured bone metabolism markers.
- Utilized differential expression analysis, Pearson correlation, and ROC curve analysis.
Main Results:
- Identified 97 upregulated and 31 downregulated miRNAs in osteoporotic women compared to controls.
- Found elevated serum levels of miR-340-5p and miR-506-3p in osteoporotic women.
- Demonstrated that miR-340-5p and miR-506-3p expressions correlate with bone metabolism markers and have high predictive value (AUC up to 0.935) for osteoporosis incidence.
Conclusions:
- Circulating miR-340-5p and miR-506-3p function as osteo-miRNAs in postmenopausal women.
- These miRNAs represent potential noninvasive biomarkers for predicting osteoporosis incidence.
- Further research can validate their clinical utility in managing postmenopausal osteoporosis.
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