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Elastomeric platform with surface wrinkling patterns to control cardiac cell alignment.

Andrew House1, Jason Cornick1, Quratulain Butt2

  • 1Otto H. York Department of Chemical and Materials Engineering, New Jersey Institute of Technology, Newark, New Jersey, USA.

Journal of Biomedical Materials Research. Part A
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Summary

Researchers developed a new method to create tunable wrinkled surfaces for 2D cardiac tissue models. This technique controls cellular alignment, crucial for understanding heart tissue development and disease.

Keywords:
biomaterialcardiac co-culturecardiac tissue modelcombinatorial culturecombinatorial platformhigh-throughputhuman cardiac fibroblastshuman cardiomyocytestissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cardiovascular Research

Background:

  • Native cardiac tissue relies on extracellular matrix (ECM) cues for proper function.
  • Cellular alignment is a key factor in cardiac tissue development, influencing cell-cell and cell-ECM interactions.
  • Developing 2D cardiac tissue models that mimic native ECM is crucial for disease modeling and drug screening.

Purpose of the Study:

  • To develop a simple and robust method for creating tunable lamellar surface wrinkling patterns.
  • To investigate the correlation between pattern dimensions (amplitude and wavelength) and cellular alignment of human cardiomyocytes (hCMs) and cardiac fibroblasts (hCFs).
  • To fabricate micro-well array devices for high-throughput screening of cardiac cell behavior on patterned surfaces.

Main Methods:

  • Fabrication of surfaces with controlled lamellar wrinkling patterns using a simple and robust approach.
  • Spatial control over pattern dimensions, including amplitude (A ≈ 2-55 μm) and wavelength (λ ≈ 35-100 μm).
  • Culturing hCMs and hCFs on patterned surfaces and analyzing cellular alignment and pattern recognition.

Main Results:

  • Cellular alignment and pattern recognition of hCMs and hCFs were found to be correlated with pattern amplitude and wavelength.
  • Fabrication of micro-well arrays with user-defined lamellar patterns for high-throughput screening.
  • Increasing cell seeding density diminished cellular alignment, while varying hCM/hCF seeding ratios allowed for co-culture systems independent of cellular alignment.

Conclusions:

  • The developed wrinkling surface fabrication method offers precise control over topographical cues for cardiac tissue engineering.
  • This platform enables systematic investigation of cell-matrix interactions and the impact of cellular alignment on cardiac cell behavior.
  • The micro-well array devices facilitate high-throughput screening for applications in cardiac disease modeling and regenerative medicine.