Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxanthine causes a 2-cell block in random-bred mouse embryos.

D Loutradis1, D John, A A Kiessling

  • 1Department of Obstetrics, Harvard Medical School, Boston, Massachusetts 02115.

Biology of Reproduction
|September 1, 1987
PubMed
Summary

Ham's F-10 medium halts mouse embryo development at the 2-cell stage due to hypoxanthine. This developmental block in early embryos is specific to random-bred mice and can be partially reversed by EDTA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gaussian process regression coupled with mRMR to predict adulterant concentration in cocaine.

Journal of pharmaceutical and biomedical analysis·2024
Same author

HNF1A gene mutations and premature ovarian failure (POF): evidence from a clinical paradigm combining MODY 3 and POF.

Hormones (Athens, Greece)·2024
Same author

Determination of the absolute intensity of the 1205 keV γ-ray emission from <sup>91</sup>Y.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2024
Same author

The gastrointestinal status of healthy adults: a <i>post hoc</i> assessment of the impact of three distinct probiotics.

Beneficial microbes·2023
Same author

Descriptive epidemiology of coronavirus disease 2019 in Nigeria, 27 February-6 June 2020.

Epidemiology and infection·2020
Same author

Adenomyosis: Disease, uterine aging process leading to symptoms, or both?

Facts, views & vision in ObGyn·2020

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Cell Culture Media

Background:

  • Ham's F-10 is a common medium for in vitro fertilization.
  • Embryo development media can significantly impact early embryonic progression.
  • Mouse models are crucial for understanding IVF and embryo development.

Purpose of the Study:

  • To investigate the cause of developmental arrest observed in embryos cultured in Ham's F-10 medium.
  • To identify specific components in Ham's F-10 responsible for blocking embryo development.
  • To compare the effects of different culture media on embryo development in different mouse strains.

Main Methods:

  • Culturing embryos from random-bred (CD-1) and hybrid-inbred (BDF1) mice in Ham's F-10 and a modified Krebs-Ringer bicarbonate medium (BWW).

Related Experiment Videos

  • Assessing embryo development to the blastocyst stage.
  • Supplementing BWW with Ham's F-10 components to pinpoint the inhibitory substance.
  • Testing the effect of ethylenediaminetetraacetic acid (EDTA) on reversing the developmental block.
  • Conducting breeding experiments to evaluate the influence of the paternal genome.

    • Main Results:

    • Ham's F-10 blocked development in over 92% of CD-1 mouse embryos at the 2-cell stage, while BDF1 embryos were unaffected.
    • BWW medium supported blastocyst development in 85% (CD-1) and 100% (BDF1) of embryos.
    • Hypoxanthine (6-30 microM) in Ham's F-10 was identified as the primary cause of the developmental block in CD-1 embryos.
    • The hypoxanthine-induced block was partially reversed (40%) by adding EDTA.
    • The paternal genome did not influence the hypoxanthine sensitivity of CD-1 embryos.

    Conclusions:

    • Hypoxanthine in Ham's F-10 medium is responsible for the 2-cell stage developmental block in random-bred mouse embryos.
    • EDTA can partially mitigate the negative effects of hypoxanthine on embryo development.
    • Embryo sensitivity to culture medium components varies between different genetic backgrounds, highlighting the importance of strain-specific media optimization.