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Comparative transcriptomic analysis reveals the important process in two rice cultivars with differences in cadmium
Shouping Zhao1, Qi Zhang1, Wendan Xiao1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Information Traceability for Agricultural Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Ecotoxicology and Environmental Safety
|February 10, 2023
Summary
Low grain cadmium (Cd) rice cultivar ZZ143 shows stronger tolerance and absorption than high grain Cd cultivar YX409. Transcriptomic analysis reveals ZZ143
Area of Science:
- Plant Science
- Environmental Science
- Genomics
Background:
- Cadmium (Cd) contamination in rice poses a significant threat to food safety and human health.
- Understanding genotypic differences in Cd accumulation is crucial for developing mitigation strategies.
- Rice cultivars exhibit varying susceptibility to Cd stress, necessitating molecular-level investigations.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying genotypic variations in Cd accumulation between two rice cultivars.
- To compare the transcriptomic responses of low-Cd (ZZ143) and high-Cd (YX409) rice cultivars under Cd stress.
- To identify key genes and pathways involved in Cd resistance and accumulation in rice.
Main Methods:
- Comparative transcriptomic profiling of two rice cultivars (ZZ143 and YX409) under 100μmol/L Cd stress.
- Differential gene expression analysis to identify differentially expressed genes (DEGs).
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.
Main Results:
- Significant numbers of DEGs were identified in both cultivars, with ZZ143 showing more enriched GO terms and KEGG pathways.
- ZZ143 demonstrated enhanced sulfur assimilation and cysteine synthesis capabilities compared to YX409.
- YX409 exhibited a stronger capacity for maintaining cell wall stability.
- DEGs related to metabolic pathways, secondary metabolite biosynthesis, hormone signaling, and MAPK signaling were identified.
Conclusions:
- The ZZ143 cultivar possesses superior Cd absorption and root tolerance compared to YX409, as indicated by transcriptomic data.
- Differential expression of genes involved in sulfur metabolism, cell wall integrity, and signaling pathways contributes to varying Cd tolerance.
- This study provides insights into the genetic basis of Cd accumulation in rice, aiding in breeding low-Cd varieties.
