A dual-functional module cellular electrochemical sensing platform for simultaneous detection guanine and xanthine

Area of Science:

• Electrochemistry
• Biomarker Detection
• Molecular Imprinting Technology

Background:

• Separating superimposed electrochemical signals of intracellular guanine (G) and xanthine (X) is challenging, hindering cell electrochemistry applications.
• Existing methods struggle with the interference between G and X signals within cells.

Purpose of the Study:

• To develop a novel dual-functional module cellular electrochemical sensing platform (DMCEP) for sensitive and interference-free detection of intracellular G and X.
• To enable simultaneous signal separation and analysis of G and X within living cells.
• To establish a foundation for electrochemical cell viability detection and simultaneous tracking of multiple biomarkers.

Main Methods:

• Construction of independent G-functional module (G-FM) and X-functional module (X-FM) using molecular imprinting technology.
• Integration of G-FM and X-FM into a DMCEP for synchronous signal transmission via two channels.
• Utilizing DMCEP to separate superimposed G and X signals within cells.

Main Results:

• DMCEP demonstrated satisfactory reproducibility (RSD 3.10% for G, 2.22% for X) and repeatability (RSD 3.72% for G, 3.05% for X).
• Achieved low detection limits of 0.05 μM for G and 0.06 μM for X.
• Established good linear relationships between cell concentrations and G/X signals over specific ranges.
• Successfully tracked MCF-7 cell growth, showing earlier detection of viability changes compared to traditional cell counting.

Conclusions:

• The developed DMCEP effectively separates and detects intracellular G and X without interference, overcoming a major obstacle in cell electrochemistry.
• This strategy provides a robust method for electrochemical cell viability detection and offers new possibilities for simultaneous monitoring of multiple intracellular analytes.
• The platform opens avenues for simultaneous recording of superimposed signals and tracking of multiple biomarkers in biological systems.