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Cost effective media optimization for PHB production by Bacillus badius MTCC 13004 using the statistical approach
Sweeta Soni1, Vinod Chhokar1, Vikas Beniwal2
1Dept. of Bio and Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar 125001, India.
International Journal of Biological Macromolecules
|February 10, 2023
Summary
This study demonstrates cost-effective production of polyhydroxybutyrate (PHB), a biodegradable plastic alternative, using agricultural waste by Bacillus badius. Optimized conditions significantly increased PHB yield, offering a viable solution to reduce plastic pollution.
Area of Science:
- Biotechnology
- Polymer Science
- Environmental Science
Background:
- Traditional plastics cause global environmental issues.
- High cost of bio-based plastics like polyhydroxybutyrate (PHB) hinders market adoption.
- Agricultural residues present an opportunity for low-cost biopolymer production.
Purpose of the Study:
- To develop a cost-effective method for producing polyhydroxybutyrate (PHB) using agricultural residues.
- To optimize PHB production by Bacillus badius MTCC 13004 through media manipulation.
- To characterize the produced PHB and its synthesis pathway.
Main Methods:
- Utilized agricultural residues (banana peel, mustard cake) as substrates for PHB production.
- Employed Box-Behnken Design (BBD) for optimizing media components (pH, Na acetate, banana peel, mustard cake).
- Characterized the produced PHB using FTIR, NMR, and thermal analysis (Tm, Tg); designed a PCR protocol for the PHB synthase gene (phbC).
Main Results:
- Achieved a PHB yield of 2.11 g/L under optimized conditions, a significant increase from 0.72 g/L.
- FTIR and NMR analyses confirmed the identity and structure of the produced PHB, matching commercial standards.
- Determined the melting temperature (Tm) at 165.14 °C and glass transition temperature (Tg) at 2.68 °C for the biopolymer.
Conclusions:
- Cost-effective PHB production is feasible using agricultural residues and Bacillus badius.
- Media optimization using BBD significantly enhances PHB yield.
- The characterized PHB is comparable to commercial PHB, indicating its potential as a sustainable plastic alternative.
