Investigating immobilization efficiency of Pb in solution and loess soil using bio-inspired carbonate precipitation

Lead (Pb) metal accumulation in surrounding environments can cause serious threats to human health, causing liver and kidney function damage. This work explored the potential of applying the MICP technology to remediate Pb-rich water bodies and Pb-contaminated loess soil sites. In the test tube experiments, the Pb immobilization efficiency of above 85% is attained through PbCO₃ and Pb(CO₃)₂(OH)₂ precipitation. Notwithstanding that, in the loess soil column tests, the Pb immobilization efficiency decreases with the increase in depth and could be as low as approximately 40% in the deep ground. PbCO₃ and Pb(CO₃)₂(OH)₂ precipitation has not been detected as the majority of Pb²⁺ combines with -OH (hydroxyl group) when subjected to 500 mg/kg Pb²⁺. The alkaline front promotes the chemisorption of Pb²⁺ with CO₃^2- reducing the depletion of quartz mineral close to the surface. However, OH^- is in shortage in the deep ground retarding the Pb immobilization. The Pb immobilization efficiency thus decreases with the increase in depth. Quartz and albite minerals, when subjected to 16,000 mg/kg Pb²⁺, appear not to intervene in the chemisorption with Pb²⁺ where the chemisorption of Pb²⁺ with CO₃^2- plays a major role in the Pb immobilization. Compared to the nanoscale urease applied to the enzyme-induced carbonate precipitation (EICP) technology, the micrometer scale ureolytic bacteria penetrate into the deep ground with difficulty. The 'size' issue remains to be addressed in near future.