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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Related Experiment Video

Updated: Aug 10, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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VIS-NIR Diffuse Reflectance Spectroscopy System with Self-Calibrating Fiber-Optic Probe: Study of Perturbation

Valeriya Perekatova1, Alexey Kostyuk1, Mikhail Kirillin1

  • 1Institute of Applied Physics RAS, 603950 Nizhny Novgorod, Russia.

Diagnostics (Basel, Switzerland)
|February 11, 2023
PubMed
Summary

Self-calibrating diffuse reflectance spectroscopy (DRS) offers superior stability against measurement errors compared to single-slope DRS. This advancement enhances the reliability of optical measurements in various applications.

Keywords:
diffuse optical spectroscopydiffuse reflectance spectroscopydiffuse scatteringoxygenationratiometric approachself-calibrating approachtissue chromophorestissue optics

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Optical Engineering

Background:

  • Diffuse reflectance spectroscopy (DRS) is crucial for non-invasive tissue analysis.
  • Traditional single-slope DRS is susceptible to instrumental perturbations.
  • Developing robust optical measurement techniques is essential for reliable in vivo studies.

Purpose of the Study:

  • To comparatively analyze the stability of self-calibrating (dual-slope) and single-slope DRS methods.
  • To evaluate their resistance to various measurement perturbations.
  • To assess performance in both phantom and in vivo settings.

Main Methods:

  • Developed a VIS-NIR fiber-optic DRS setup with dual source and detection fibers.
  • Implemented both single-slope and self-calibrating measurement approaches.
  • Tested stability against channel attenuations, fiber bending, and probe-tissue interface variations.

Main Results:

  • Self-calibrating DRS showed high resistance to perturbations in both source and detection channels.
  • Single-slope DRS demonstrated resistance only to source channel perturbations.
  • The self-calibrating method proved more robust in phantom and human palm studies.

Conclusions:

  • Self-calibrating DRS offers significant advantages in stability and reliability over single-slope DRS.
  • This technique is better suited for applications requiring high accuracy under challenging conditions.
  • The findings support the adoption of self-calibrating DRS for improved optical measurements.