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Characterization of Stress Granule Protein Turnover in Neuronal Progenitor Cells Using Correlative STED and NanoSIMS
Stefania Rabasco1, Alicia A Lork1, Emmanuel Berlin1
1Department of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
International Journal of Molecular Sciences
|February 11, 2023
Summary
Stress granules (SGs) form dynamic protein hubs during cellular stress. ER stress impairs protein turnover, hindering recovery even after stress cessation, impacting cell survival and neurodegenerative disease research.
Area of Science:
- Cell Biology
- Neuroscience
- Biochemistry
Background:
- Stress granules (SGs) are crucial for cell survival under stress.
- Dysfunctional SGs are implicated in neurodegenerative diseases.
- Mechanisms of SG assembly/disassembly and cellular recovery remain unclear.
Purpose of the Study:
- To investigate the molecular dynamics of SG formation and protein turnover in neuronal progenitor cells (NPCs) under ER stress.
- To understand the impact of ER stress on cellular protein turnover and recovery.
Main Methods:
- Correlative STED and NanoSIMS imaging were used to visualize SGs and quantify protein turnover.
- NPCs were cultured with 15N-leucine and treated with thapsigargin (TG) for ER stress induction.
Main Results:
- TG-induced SGs are dynamic and recruit cytoplasmic constituents.
- ER stress significantly impairs overall cellular protein turnover.
- Impaired protein turnover persisted after the stress recovery period.
Conclusions:
- ER stress disrupts normal protein turnover dynamics, affecting cellular recovery.
- SGs play a role in managing protein homeostasis during stress.
- Further research is needed to elucidate SG roles in neurodegeneration and cellular resilience.
