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Updated: Aug 10, 2025
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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
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Significance of Singlet Oxygen Molecule in Pathologies
Kazutoshi Murotomi1, Aya Umeno2, Mototada Shichiri3
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan.
International Journal of Molecular Sciences
|February 11, 2023
Summary
Singlet oxygen contributes to disease and aging by causing oxidative stress. Measuring singlet oxygen-induced oxidation products may offer new diagnostic biomarkers for conditions like glaucoma and diabetes.
Area of Science:
- Biochemistry
- Molecular Biology
- Pathophysiology
Background:
- Reactive oxygen species, including singlet oxygen, are implicated in disease onset, progression, and aging.
- Singlet oxygen is generated through various chemical, photochemical, and enzymatic reactions, contributing to oxidative stress.
- Oxidative stress from singlet oxygen leads to detrimental modifications of biomolecules like proteins, DNA, and lipids.
Purpose of the Study:
- To review the molecular mechanisms of singlet oxygen production in vivo.
- To discuss methods for evaluating singlet oxygen-induced damage.
- To explore the role of singlet oxygen in skin and eye diseases and its potential as a diagnostic biomarker.
Main Methods:
- Review of literature on singlet oxygen production and damage evaluation.
- Analysis of lipid oxidation products from singlet oxygen-mediated oxidation in disease models.
- Discussion of clinical relevance and potential biomarker applications.
Main Results:
- Singlet oxygen-mediated oxidation products were identified in glaucoma, early diabetes, and asthma models.
- These oxidation products have not been clinically measured in these diseases.
- Carotenoids are highlighted as potential singlet oxygen scavengers for disease prevention.
Conclusions:
- Singlet oxygen plays a significant role in disease pathogenesis through oxidative stress.
- Oxidation products induced by singlet oxygen show promise as novel diagnostic biomarkers.
- Targeting singlet oxygen with scavengers like carotenoids may offer therapeutic benefits.
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