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Updated: Aug 10, 2025

Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
The Ubiquitin-26S Proteasome Pathway and Its Role in the Ripening of Fleshy Fruits
Wen Jia1, Gangshuai Liu1, Peiyu Zhang1
1Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Abstract:
The 26S proteasome is an ATP-dependent proteolytic complex in eukaryotes, which is mainly responsible for the degradation of damaged and misfolded proteins and some regulatory proteins in cells, and it is essential to maintain the balance of protein levels in the cell. The ubiquitin-26S proteasome pathway, which targets a wide range of protein substrates in plants, is an important post-translational regulatory mechanism involved in various stages of plant growth and development and in the maturation process of fleshy fruits. Fleshy fruit ripening is a complex biological process, which is the sum of a series of physiological and biochemical reactions, including the biosynthesis and signal transduction of ripening related hormones, pigment metabolism, fruit texture changes and the formation of nutritional quality. This paper reviews the structure of the 26S proteasome and the mechanism of the ubiquitin-26S proteasome pathway, and it summarizes the function of this pathway in the ripening process of fleshy fruits.
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