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Transcriptome-Based Traits of Radioresistant Sublines of Non-Small Cell Lung Cancer Cells

Margarita Pustovalova1, Philipp Malakhov1, Anastasia Guryanova1

  • 1School of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia.

International Journal of Molecular Sciences
|February 11, 2023
PubMed

Insights

This study explored how non-small cell lung cancer (NSCLC) cells become resistant to radiation therapy. Researchers identified specific gene expression changes that may offer new therapeutic targets for improving NSCLC treatment outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Radioresistance presents a significant challenge in treating non-small cell lung cancer (NSCLC).
  • Understanding the molecular mechanisms underlying NSCLC radioresistance is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the transcriptional alterations in radioresistant NSCLC cells.
  • To identify key genes and molecular pathways associated with radioresistance in NSCLC.
  • To establish radioresistant subclones of NSCLC cell lines for further study.

Main Methods:

  • Establishment of radioresistant subclones from p53 wild-type (A549) and p53-deficient (H1299) NSCLC cell lines.
  • RNA sequencing (RNA-seq) to analyze global gene expression changes.
  • Utilizing the Oncobox bioinformatics platform to quantify pathway activation differences.

Main Results:

  • In p53 wild-type A549 cells, 322 genes were differentially expressed after irradiation.
  • In p53-deficient H1299 cells, 1628 genes and 1616 pathways showed altered expression post-irradiation.
  • Significant transcriptional reprogramming occurs in NSCLC cells upon development of radioresistance.

Conclusions:

  • Gene expression patterns in radioresistant NSCLC cells reflect complex biological processes.
  • Identified genes and pathways may serve as potential biomarkers for NSCLC radioresistance.
  • These findings suggest novel therapeutic targets for overcoming radioresistance in NSCLC treatment.
Keywords:
DNA repairgene expressionionizing radiationnon-small cell lung cancerradioresistancetranscriptomics

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