Related Experiment Video
Updated: Jul 6, 2026
10:51
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
Published on: August 30, 2016
22.6K
Culturing the Chicken Intestinal Microbiota and Potential Application as Probiotics Development
Ke Ma1, Wei Chen1, Xiao-Qi Lin1
1Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130012, China.
International Journal of Molecular Sciences
|February 11, 2023
Summary
This study isolated Limosilactobacillus reuteri strain YPG14 from chicken intestines, demonstrating its probiotic potential. The strain effectively combats Salmonella Pullorum infection in chicks, improving survival and reducing bacterial load.
Area of Science:
- Microbiology
- Animal Science
- Probiotics
Background:
- Gut microbiota plays a crucial role in host health and can be a source of probiotics.
- Isolation and characterization of specific microbial species are essential for understanding their functions and developing novel applications.
Purpose of the Study:
- To isolate and identify chicken intestinal bacteria with potential probiotic properties.
- To evaluate the efficacy of Limosilactobacillus reuteri strain YPG14 as a probiotic against Salmonella Pullorum infection in poultry.
Main Methods:
- Isolation and identification of 507 chicken intestinal bacterial isolates.
- Antibacterial activity testing against Salmonella Pullorum.
- Acid tolerance, simulated intestinal fluid stability, auto-aggregation, and mucin binding assays for Limosilactobacillus reuteri strain YPG14.
- In vivo efficacy study in chicks challenged with Salmonella Pullorum.
Main Results:
- 63 Lactobacillus strains showed antibacterial activity against Salmonella Pullorum.
- Limosilactobacillus reuteri strain YPG14 demonstrated strong acid tolerance, stability in simulated intestinal fluid, and good auto-aggregation and mucin binding capabilities.
- In vivo, Limosilactobacillus reuteri strain YPG14 significantly improved chick survival rates, reduced Salmonella Pullorum loads, and alleviated intestinal damage.
Conclusions:
- Limosilactobacillus reuteri strain YPG14 possesses significant probiotic properties.
- This strain shows promise for development as a novel probiotic agent in the poultry industry to combat Salmonella Pullorum infections.
Related Concept Videos
01:22Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
01:24iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
01:30Development of Human Microbiota
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...
01:27Microbiota of the Stomach and Small Intestine
The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
01:27Microbiota of the Large Intestine
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
01:21Microbiota Modulation by Antibiotics
Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...