Photo-Fenton and TiO₂ Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and...

Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton's reagent and TiO₂, respectively. A comprehensive study of homogeneous and heterogeneous photocatalysis on a range of microorganisms will significantly establish the most efficient method. Here, we report a comparative study of TiO₂- and Fe⁺³-based photocatalytic inactivation under UV-A of diverse microorganisms, including Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, bacterial spores (Bacillus stearothermophilus spores) and viruses (MS2). We also present data on the optimization of TiO₂ photocatalysis, including optimal catalyst concentration and H₂O₂ supplementation. Our results indicate that both photo-Fenton and TiO₂ could be successfully applied for the management of microbial loads in liquids. Efficient microorganism inactivation is achieved with homogeneous photocatalysis (7 mg/L Fe⁺³, 100 mg/L H₂O₂, UV-A) in a shorter processing time compared to heterogeneous photocatalysis (0.5 g/L TiO₂, UV-A), whereas similar or shorter processing is required when heterogenous photocatalysis is performed using microorganism-specific optimized TiO₂ concentrations and H₂O₂ supplementation (100 mg/L); higher H₂O₂ concentrations further enhance the heterogenous photocatalytic inactivation efficiency. Our study provides a template protocol for the design and further application for large-scale photocatalytic approaches to inactivate pathogens in liquid biomedical waste.