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Endophytic Fungi Isolated from Ageratina adenophora Exhibits Potential Antimicrobial Activity against

Juan Wen1, Samuel Kumi Okyere1,2, Jianchen Wang1

  • 1Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

Plants (Basel, Switzerland)
|February 11, 2023
PubMed

Insights

Novel endophytic fungi from Ageratina adenophora show antibacterial activity against multidrug-resistant bacteria like methicillin-resistant Staphylococcus aureus (MRSA). These findings highlight potential new sources for developing urgently needed antibiotics.

Area of Science:

  • Microbiology
  • Pharmacology
  • Mycology

Background:

  • Multidrug-resistant bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), pose a significant global health threat due to limited treatment options.
  • Endophytic fungi represent a promising, yet largely untapped, reservoir for discovering novel bioactive compounds with therapeutic potential.

Purpose of the Study:

  • To isolate and identify endophytic fungi from Ageratina adenophora with antibacterial activity against multidrug-resistant bacteria.
  • To evaluate the antibacterial and bactericidal efficacy of extracts from these endophytic fungi.

Main Methods:

  • Fungal isolation from Ageratina adenophora using morphological and ITS-rDNA sequencing for identification.
  • Determination of fungal diversity indices (Species richness, Shannon-Wiener, Simpson's).
  • Agar well diffusion assays and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for ethyl acetate extracts.

Main Results:

  • A total of 124 morphotype strains were identified, indicating abundant endophytic fungal resources in A. adenophora.
  • Ethyl acetate extracts from Penicillium sclerotigenum, Diaporthe kochmanii, and Pestalotiopsis trachycarpicola exhibited moderate antibacterial and bactericidal activity against MRSA SMU3194.
  • MIC values ranged from 0.5-1 mg/mL, and MBC values ranged from 1-2 mg/mL.

Conclusions:

  • Ageratina adenophora harbors diverse endophytic fungi with significant potential for pharmacological applications.
  • Specific endophytic fungi, including Penicillium sclerotigenum, Diaporthe kochmanii, and Pestalotiopsis trachycarpicola, are promising sources for developing novel antibacterial agents against MRSA.
Keywords:
Ageratina adenophoraLC-MS analysisantibacterial activityendophytic fungisecondary metabolites

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