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Updated Overview of TALEN Construction Systems
Tetsushi Sakuma1, Takashi Yamamoto2
1Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan. tetsushi-sakuma@hiroshima-u.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2023
Summary
Transcription activator-like effector nucleases (TALENs) offer customizable genome editing by modularly assembling DNA-binding repeats. This chapter reviews efficient TALEN construction systems and available resources for researchers.
Area of Science:
- Molecular Biology
- Gene Editing Technologies
Background:
- Transcription activator-like effector nucleases (TALENs) are second-generation genome editing tools.
- TALENs combine customizable DNA-binding repeats with the FokI nuclease domain.
- Each repeat in a TALEN recognizes a single DNA base.
Approach:
- This chapter focuses on methods for assembling TALEN DNA-binding repeat arrays.
- It reviews established systems like Golden Gate assembly, serial ligation, and ligation-independent cloning.
- The availability of reagents and protocols for these systems is also discussed.
Key Points:
- Efficient assembly of repetitive DNA-binding repeat arrays is crucial for TALEN construction.
- Various molecular cloning techniques facilitate TALEN system development.
- Updated information on systems, reagents, and protocols aids researchers in selecting optimal methods.
Conclusions:
- This review provides a comprehensive overview of TALEN construction systems.
- It aims to guide researchers in choosing the most suitable methods for their specific applications.
- The goal is to facilitate the broader utilization of TALENs across diverse research fields.
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