Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

105
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
105
CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.2K
Homologous Recombination02:31

Homologous Recombination

50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

79
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
79

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmental genetic determinants of the human cerebrospinal fluid-ventricular system.

Science translational medicine·2026
Same author

Inhibition of ceramide synthase 5 ameliorates pulmonary fibrosis via suppression of TGF-β/Smad signaling.

Journal of pharmacological sciences·2026
Same author

An Adnp frameshift variant disrupts Wnt signalling inducing chromatocytoskeletal defects and autism-related behaviour in male mice.

EBioMedicine·2026
Same author

Reactive sulfur species are inactivated and excreted as trimethylsulfonium ion by thiopurine S-methyltransferase.

Redox biology·2026
Same author

Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity.

eLife·2026
Same author

Germline epigenome editing identifies H3K9me3 as a mediator of intergenerational DNA methylation recovery in mice.

Nature communications·2025

Related Experiment Video

Updated: Aug 10, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.2K

CRISPR/Cas9.

Izuho Hatada1,2, Sumiyo Morita3, Takuro Horii3

  • 1Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan. hatada@gunma-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2023
PubMed
Summary

The CRISPR/Cas9 system offers a simple and widely adopted method for genome editing. This study details vector construction and target gene editing in cells using this powerful technology.

Keywords:
CRISPR/Cas9Cas9gRNA

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.7K
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K

Related Experiment Videos

Last Updated: Aug 10, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.2K
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.7K
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • CRISPR/Cas9 is a revolutionary genome-editing tool.
  • Its simplicity and ease of use have led to global adoption.
  • Efficient genome editing is crucial for biological research and therapeutic development.

Purpose of the Study:

  • To describe the construction of vectors for the CRISPR/Cas9 system.
  • To demonstrate genome editing of a target gene in cells.
  • To provide a practical guide for utilizing CRISPR/Cas9 technology.

Main Methods:

  • Vector construction for CRISPR/Cas9 components.
  • Introduction of CRISPR/Cas9 components into target cells.
  • Verification of successful genome editing at the target locus.

Main Results:

  • Successfully constructed functional CRISPR/Cas9 vectors.
  • Demonstrated efficient editing of the target gene in cellular models.
  • Validated the precision and efficacy of the CRISPR/Cas9 system.

Conclusions:

  • The described methods facilitate straightforward CRISPR/Cas9 genome editing.
  • This approach is valuable for researchers studying gene function.
  • CRISPR/Cas9 technology holds significant potential for various applications.