Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

105
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
105
CRISPR01:59

CRISPR

52.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment and characterization of adap1-deficient zebrafish.

Development, growth & differentiation·2025
Same author

Systematic Comparison of Computational Tools for Sanger Sequencing-Based Genome Editing Analysis.

Cells·2024
Same author

Efficient detection of single nucleotide variants in targeted genomic loci.

Development, growth & differentiation·2024
Same author

Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis.

Scientific reports·2023
Same author

Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9.

Nucleic acids research·2022
Same author

Involvement of the zebrafish trrap gene in craniofacial development.

Scientific reports·2021

Related Experiment Video

Updated: Aug 10, 2025

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.3K

CRISPR-Cas9-Mediated Genome Modifications in Zebrafish.

Yusuke Kamachi1, Atsuo Kawahara2

  • 1School of Environmental Science and Engineering, Kochi University of Technology, Tosayamada-cho, Kami, Kochi, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2023
PubMed
Summary

CRISPR-Cas9 gene editing in zebrafish now allows precise DNA insertions (knock-in) using microhomology-mediated end joining or single-stranded DNA donors, overcoming previous limitations in targeted gene modification.

Keywords:
CRISPR-Cas9HDRKnock-inKnockoutLong ssDNAMMEJNHEJZebrafish

More Related Videos

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.5K
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.1K

Related Experiment Videos

Last Updated: Aug 10, 2025

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.3K
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.5K
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Zebrafish Models

Background:

  • CRISPR-Cas9 technology enables genetic modifications in zebrafish.
  • The system efficiently causes gene knockouts via DNA double-strand breaks (DSBs).
  • Targeted DNA insertion (knock-in) remains challenging with standard CRISPR-Cas9.

Purpose of the Study:

  • To summarize CRISPR-Cas9-mediated genome modification strategies in zebrafish.
  • To highlight advancements in targeted gene knock-in techniques.
  • To present methods for precise exogenous DNA integration.

Main Methods:

  • Utilizing CRISPR-Cas9 to induce targeted DSBs.
  • Employing microhomology-mediated end joining (MMEJ) for precise gene integration.
  • Using long single-stranded DNA (ssDNA) donors for knock-in of short sequences.

Main Results:

  • Established MMEJ-mediated targeted integration of exogenous genes in zebrafish.
  • Developed a method for knocking in ~200 nt sequences using ssDNA donors.
  • Demonstrated successful CRISPR-Cas9-based genome modification strategies.

Conclusions:

  • CRISPR-Cas9 genome editing in zebrafish has advanced significantly.
  • New methods enable precise targeted gene knock-in, overcoming previous limitations.
  • These strategies offer powerful tools for zebrafish genetic research.