Novel ultrasensitive Raman assay method based on enzyme mimetics for ultra trace of H₂O₂

Enzyme mimetics have been widely applied on H₂O₂ assay, but it is still challenging and interesting to realize the sensitive detection for ultra-trace H₂O₂. Here, an ultrasensitive Raman assay method based on novel WO₃@IP6-Fe³⁺ enzyme mimetics with peroxidase-like activity was established. WO₃ microspheres (MSs) were found to have weak peroxidase-like activity, and the combination of IP6-Fe³⁺ and WO₃ can produce stronger activity. WO₃@IP6-Fe³⁺ MSs showed polyhedron-like structure, uniform size, and smooth surface. Although WO₃@IP6-Fe³⁺ enzyme mimetics have low catalytic efficiency and high absorbance background, the proposed Raman method can bypass the above problems. In Raman method, high concentration of WO₃@IP6-Fe³⁺ can be used to overcome low catalytic efficiency without high absorbance background. Moreover, 3,3',5,5'-tetramethylbenzidine oxide has prominent characteristic Raman peak at 1608 cm^-1, greatly improving the sensitivity and eliminating interference of impurities. Due to the high sensitivity and low background, Raman assay showed the ultra-low limit of detection (5.49 × 10^-15 M), which was 4-7 orders of magnitude lower than other detection methods. The ultrasensitive Raman assay not only provided the possibility for the enzyme mimetics-based detection of ultra-trace H₂O₂, but also enable the enzyme mimetics with low activity to be applied.