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Related Experiment Videos

Evaluation of the Hitachi 704 automatic analyzer.

E G Lentjes1, G A Harff, E T Backer

  • 1Department of Clinical Chemistry, Diaconessenhuis, Leiden, The Netherlands.

Clinical Chemistry
|November 1, 1987
PubMed
Summary

The Hitachi 704 automatic analyzer demonstrates good analytical performance, with precise photometric and probe imprecision. However, reagent carryover was detected in specific assay sequences.

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Area of Science:

  • Clinical chemistry and laboratory diagnostics
  • Analytical instrumentation performance evaluation

Background:

  • The Hitachi 704 automatic analyzer is a widely used platform in clinical laboratories.
  • Understanding its analytical performance is crucial for reliable diagnostic testing.

Purpose of the Study:

  • To comprehensively evaluate the analytical performance characteristics of the Hitachi 704 automatic analyzer.
  • To assess key parameters including linearity, photometric and volumetric imprecision, temperature control, carryover, and overall test accuracy.

Main Methods:

  • Spectrophotometric linearity was assessed at 340 nm.
  • Photometric imprecision was measured bichromatically at 340 and 376 nm across various absorbance levels.
  • Sample and reagent probe imprecision and volume delivery accuracy were evaluated.
  • Cuvet temperature stability was monitored using cresol red spectrophotometry.
  • Sample and reagent carryover were investigated using specific assay combinations.
  • Imprecision for nine clinical chemistry tests was determined at three concentration levels.
  • Method comparison was performed against the SMAC II reference method.

Main Results:

  • The spectrophotometer exhibited linearity up to 2.8 A at 340 nm.
  • Photometric imprecision was low, ranging from 0.14% to 0.49% across different absorbance values.
  • Sample probe imprecision was 0.4%, with minor volume delivery deviations (-2.4% to -4.4%).
  • Reagent probe imprecision ranged from 0.14% to 0.29%, with volume delivery deviations from +1.7% to +4.4%.
  • Cuvet temperature was stable at 29.8°C (SD 0.05°C).
  • No sample carryover was detected; however, reagent carryover occurred between specific assays (bilirubin/total protein, LDH/ALT).
  • Test imprecision ranged from 1.1% to 4.4%.
  • Method comparison showed good agreement with the SMAC II.
  • Overall precision was superior to the Hitachi 705 analyzer.

Conclusions:

  • The Hitachi 704 automatic analyzer demonstrates robust analytical performance suitable for routine clinical laboratory use.
  • While generally reliable, specific reagent carryover requires careful consideration in assay sequencing.
  • The analyzer offers improved precision compared to previous models like the Hitachi 705.

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