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Dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway
Jinpeng Yao1, Yuanhua Cai2, Zhixiang Chen1
1Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China.
Genomics
|February 12, 2023
Summary
DEAH-box helicase 15 (DHX15) is crucial for zebrafish intestinal development. Knockout of dhx15 causes severe intestinal defects by down-regulating the Wnt signaling pathway.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- DEAH-box helicase 15 (DHX15) is an ATP-dependent RNA helicase involved in RNA metabolism.
- Emerging evidence suggests DHX15 plays a role in intestinal immunity.
- The specific function of DHX15 in intestinal development remains largely unknown.
Purpose of the Study:
- To investigate the role of DHX15 in zebrafish intestinal development.
- To elucidate the molecular mechanisms underlying DHX15's function in the intestine.
Main Methods:
- Generation of dhx15 knockout zebrafish.
- Assessment of intestinal morphology and cell proliferation/apoptosis.
- Genome-wide RNA analysis and quantitative reverse transcription PCR (qRT-PCR).
Main Results:
- dhx15 knockout zebrafish exhibited profound intestinal defects.
- Significant decreases in intestinal cell proliferation and increases in apoptosis were observed.
- RNA analysis revealed down-regulation of the Wnt signaling pathway in dhx15 knockout models.
Conclusions:
- DHX15 plays a critical, previously unidentified role in regulating zebrafish intestinal development.
- DHX15 regulates intestinal development primarily through modulation of the Wnt signaling pathway.
- This study expands the known functions of DHX15 beyond its established role in intestinal immunity.
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