[Revealing the Effect of Saline Water Drip Irrigation on Soil Microorganisms in Cotton Fields Based on Metagenomics]

Saline water irrigation has become an important means to alleviate the shortage of freshwater in arid areas. However, long-term saline water irrigation can cause soil salinity accumulation, affect soil microbial community structure, and then affect soil nutrient transformation. In this study, we used metagenomics to investigate the effects of long-term saline water drip irrigation on soil microbial community structure in a cotton field. In the experiment, the salinity of irrigation water (ECw) was set to two treatments:0.35 dS·m^-1 and 8.04 dS·m^-1 (denoted as FW and SW, respectively), and the nitrogen application rates were 0 kg·hm^-2and 360 kg·hm^-2 (denoted as N0 and N360, respectively). The results showed that saline water irrigation increased soil water content, salinity, organic carbon, and total nitrogen content and decreased soil pH and available potassium content. Nitrogen fertilizer application increased soil organic carbon, salinity, and total nitrogen content and decreased soil water content, pH, and available potassium content. The dominant bacterial phyla in each treatment were:Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, and Gemmatimonadetes. Saline water irrigation significantly increased the relative abundances of Actinobacteria, Chloroflexi, Gemmatimonadetes, and Firmicutes but significantly decreased the relative abundances of Proteobacteria, Acidobacteria, Cyanobacteria, and Nitrospira. Nitrogen fertilizer application significantly increased the relative abundances of Chloroflexi and Nitrospira but significantly decreased the relative abundances of Acidobacteria, Gemmatimonadetes, Planctomycetes, Cyanobacteria, and Verrucomicrobia. LEfSe analysis showed that saline water irrigation had no significant effect on the number of potential biomarkers, and nitrogen fertilizer application decreased the number of potential biomarkers in soil microbial communities. The correlation network diagram showed that the 20 genera had different degrees of correlation, including 44 positive correlations and 48 negative correlations. The core species in the network diagram were Nocardioides, Streptomyces, Pyrinomonas, Candidatus_Solibacter, and Bradyrhizobium spp. Saline water irrigation increased the relative abundances of the denitrification genes nirK, nirS, nasB, and norC and decreased the relative abundances of the nitrification genes amoB, amoC, and nxrA, whereas nitrogen fertilizer application increased the relative abundances of the nitrification genes amoA, amoB, amoC, hao, and nxrA and decreased the relative abundances of the denitrifying genes narB, napA, nasA, and nosZ. Saline water irrigation could adversely affect soil physicochemical properties; SWC, EC_1:5, and BD were the main driving factors affecting soil microbial community structure and function genes; and soil microorganisms adapted to soil salt stress by regulating species composition.