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Related Experiment Video

Updated: Aug 10, 2025

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
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Human plasma ricinine quantification by LC-HRMS after micro-solid-phase elution.

Pauline Thiebot1,2, Nada Maaziz1, Chrystelle Oppon1

  • 1Laboratoire de Toxicologie Biologique, Fédération de Toxicologie, Hôpital Lariboisière, APHP, Paris, France.

Biomedical Chromatography : BMC
|February 13, 2023
PubMed
Summary
This summary is machine-generated.

A new LC-HRMS method rapidly and accurately quantifies ricinine in plasma. This technique is crucial for identifying ricin poisoning victims in emergencies.

Keywords:
humanintoxicationmicro-elutionricinricinine

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Area of Science:

  • Analytical Chemistry
  • Forensic Toxicology

Background:

  • Ricin is a highly toxic plant-derived protein.
  • Accurate detection of ricinine, a ricin metabolite, is vital for diagnosing ricin exposure.
  • Existing methods may lack the speed or sensitivity required for emergency situations.

Purpose of the Study:

  • To develop and validate a rapid, sensitive, and specific analytical method for ricinine.
  • To enable timely identification and quantification of ricinine in human plasma.
  • To support forensic investigations and clinical management of ricin poisoning.

Main Methods:

  • Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS) was employed.
  • Micro-solid-phase elution was used for plasma extraction, minimizing sample processing time.
  • Deuterated ricinine served as the internal standard for accurate quantification.

Main Results:

  • The method demonstrated excellent linearity (0.25–500.0 ng/mL) and accuracy (within ±5.6%).
  • High precision was achieved, with a maximum of 19.9% at the lower limit of quantification.
  • Validation confirmed selectivity, minimal carryover, acceptable matrix effects, and good stability.

Conclusions:

  • The developed LC-HRMS method is suitable for rapid and reliable ricinine detection in plasma.
  • This assay can aid in identifying victims of ricin exposure, such as in poisoning incidents or potential bioterrorism events.
  • Case reports illustrate the method's practical application in real-world intoxication scenarios.