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Related Experiment Videos

Skin absorption from patch test systems.

H O Kim1, R C Wester, J A McMaster

  • 1Department of Dermatology, University of California, San Francisco 94143.

Contact Dermatitis
|September 1, 1987
PubMed
Summary
This summary is machine-generated.

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Skin absorption of paraphenylenediamine (PPDA) varies significantly between patch test systems. Choosing the wrong system can lead to false negative results in skin toxicology testing.

Area of Science:

  • Dermatology
  • Toxicology
  • Pharmacokinetics

Background:

  • Topical drug development necessitates rigorous skin toxicology testing.
  • Various patch test systems exist for applying chemicals to the skin.

Purpose of the Study:

  • To evaluate and compare the skin absorption of paraphenylenediamine (PPDA) across different patch test systems.

Main Methods:

  • Radiolabeled [14C]-PPDA was applied to guinea pig skin using multiple patch systems at a standardized concentration (2 mg/mm²).
  • Skin absorption was quantified by measuring urinary excretion of 14C.

Main Results:

  • A significant six-fold difference in PPDA skin absorption was observed across the tested systems (p < 0.02).
  • The Hill Top Chamber showed the highest absorption (53.4%), while the AL-Test Chamber showed the lowest (8.0%).

Related Experiment Videos

  • Absorption rates decreased in the order: Hill Top > Teflon Control > Small Finn (paper) > Small Finn > Large Finn > AL-Test.

    • Conclusions:

    • The selection of a patch test system critically impacts the measured skin absorption of PPDA.
    • Inhibitory effects of certain patch systems on skin absorption could lead to false negative toxicology findings.