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Related Experiment Video

Updated: Aug 10, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Bond-Selective Full-Field Optical Coherence Tomography.

Haonan Zong, Celalettin Yurdakul, Jian Zhao

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    |February 13, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces bond-selective full-field optical coherence tomography (BS-FF-OCT), a novel technique for label-free, 3D chemical imaging. BS-FF-OCT provides detailed tomographic and chemical information from scattering biological and material samples.

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    Area of Science:

    • Biomedical Optics
    • Spectroscopy
    • Materials Science

    Background:

    • Optical coherence tomography (OCT) is a vital non-invasive 3D imaging technique in biology and medicine.
    • Existing OCT methods lack chemical specificity, limiting their diagnostic and research capabilities.
    • There is a need for imaging modalities that combine high-resolution 3D structural information with chemical identification.

    Approach:

    • Developed bond-selective full-field OCT (BS-FF-OCT) utilizing a pulsed mid-infrared laser and photothermal effect.
    • BS-FF-OCT modulates the OCT signal to achieve label-free, bond-selective 3D imaging.
    • Demonstrated the technique on various samples, including polymer beads, surgical mask fibers, cancer spheroids, C. elegans, and mouse brain slices.

    Key Points:

    • Achieved label-free, bond-selective 3D imaging of highly scattering samples.
    • Successfully imaged polymer microspheres, polypropylene fibers, biological cells, nematodes, and brain tissue.
    • Showcased 3D hyperspectral imaging capabilities and adaptability to different sample types and thicknesses.

    Conclusions:

    • BS-FF-OCT offers a powerful new tool for label-free chemical and structural 3D imaging.
    • The technique expands the applicability of OCT by providing chemical information.
    • BS-FF-OCT holds significant potential for advancements in biological research and clinical diagnostics.