Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel Chitosan-Gelatin Scaffold and Cell Spray Therapy for Treating Limbal Stem Cell Deficiency.

Macromolecular bioscience·2026
Same author

CRISPR/Cas9-mediated knockout of TopBP1 shifts the Bax/Bcl-2 balance toward apoptosis in MCF7 breast cancer cells.

Molecular biology reports·2026
Same author

Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis.

Experimental eye research·2026
Same author

Antibacterial Potential of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Against <i>Clostridium Perfringens</i>: An Experimental <i>In Vitro</i> and <i>In Silico</i> Study.

Iranian journal of medical sciences·2026
Same author

Photo-crosslinked methacrylated hyaluronic acid-iPRF hydrogel-based microneedles for accelerated wound repair.

International journal of biological macromolecules·2026
Same author

Human umbilical cord mesenchymal stem cells derived exosomes enhance the therapeutic efficacy of anti-miR-10b in glioblastoma.

Scientific reports·2026

Related Experiment Video

Updated: Aug 10, 2025

Author Spotlight: Isolating Biomolecules from Mouse Tears &#8212; A Methodology for Molecular Analysis and Biomarker Research
09:04

Author Spotlight: Isolating Biomolecules from Mouse Tears — A Methodology for Molecular Analysis and Biomarker Research

Published on: July 19, 2024

1.6K

Novel RNA extraction method from human tears.

Mahintaj Dara1, Azam Habibi2, Negar Azarpira3

  • 1Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Molecular Biology Research Communications
|February 13, 2023
PubMed
Summary

Researchers developed a novel method for direct RNA extraction from human tears. This easy protocol yields high-quality and high-quantity RNA for disease diagnosis and biomarker discovery.

Keywords:
BiomarkersExtraction methodHuman tearsRNA

More Related Videos

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.2K
Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
10:32

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Published on: August 14, 2013

7.9K

Related Experiment Videos

Last Updated: Aug 10, 2025

Author Spotlight: Isolating Biomolecules from Mouse Tears &#8212; A Methodology for Molecular Analysis and Biomarker Research
09:04

Author Spotlight: Isolating Biomolecules from Mouse Tears — A Methodology for Molecular Analysis and Biomarker Research

Published on: July 19, 2024

1.6K
Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.2K
Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
10:32

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Published on: August 14, 2013

7.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Ophthalmology

Background:

  • Human tears offer a noninvasive source for genetic material and biomarkers.
  • Tear-based biomarkers are valuable for diagnosing ocular and non-ocular diseases.
  • Existing RNA extraction methods may not be optimal for tear samples.

Purpose of the Study:

  • To develop a novel, direct RNA extraction protocol from human tears.
  • To ensure high quality and quantity of extracted RNA for downstream applications.
  • To establish an efficient method for tear-derived genetic material analysis.

Main Methods:

  • A modified TRIzol-based protocol was developed for direct RNA extraction.
  • RNA quality was assessed using the 260/280 UV absorption ratio via Nanodrop.
  • RNA quantity and yield were validated using real-time PCR.

Main Results:

  • The developed method consistently yielded high-quality RNA (260/280 ratio of 1.8-2.0).
  • An average yield of 95 μg of total RNA was obtained from human tear samples.
  • The protocol proved effective for direct RNA isolation from tears.

Conclusions:

  • A simple and effective method for direct total RNA extraction from human tears was established.
  • This protocol provides high-quality and high-quantity RNA suitable for diagnostic purposes.
  • The method facilitates the use of tears as a valuable source for biomarker research.