Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intra-CNS AAV9-<i>GBA1</i> delivery yields species and route of administration differences in safety and transgene expression.

Molecular therapy. Advances·2026
Same author

Immunotherapy with B28, an antibody to Aβ oligomers, potently decreases amyloid plaques, microgliosis, and memory decline in APP knock-in mice.

Cell reports·2026
Same author

Retraction notice to "Cognitive effects of cell-derived and synthetically-derived Aβ oligomers" [Neurobiol. Aging 32 (2009) 1784-1794].

Neurobiology of aging·2026
Same author

Novel Monoclonal Antibody Detects Small Aβ Oligomers More Sensitively Than Lecanemab in Alzheimer's Disease CSF, Serum and Culture Media.

Annals of neurology·2026
Same author

Identification of aryl hydrocarbon receptor as a functional target that enhances astrocytic ApoE secretion.

Cell chemical biology·2026
Same author

Brain Endothelial Soluble ST2 Production and Cerebral Edema in a Rat Model of Ischemic Stroke.

Annals of neurology·2025

Related Experiment Video

Updated: Aug 10, 2025

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.2K

Methods for the isolation and analysis of Aβ from postmortem brain.

Wei Hong1,2, Wen Liu1, Alexandra O Desousa1

  • 1Laboratory for Neurodegenerative Research, Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, United States.

Frontiers in Neuroscience
|February 13, 2023
PubMed
Summary

Researchers developed new methods to study amyloid beta-protein (Aβ) from human brains, focusing on disease-related Aβ dimers and their neurotoxic effects in Alzheimer's disease (AD). This platform aids in identifying mitigation strategies.

Keywords:
amyloid plaquesamyloid β-proteininduced neuronsneurotoxicitysoluble aggregates

More Related Videos

Detecting Amyloid-&#946; Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

3.4K
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
07:28

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Published on: May 19, 2022

3.9K

Related Experiment Videos

Last Updated: Aug 10, 2025

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.2K
Detecting Amyloid-&#946; Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

3.4K
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
07:28

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Published on: May 19, 2022

3.9K

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Alzheimer's disease (AD) pathogenesis is linked to amyloid beta-protein (Aβ).
  • Prior research predominantly used synthetic Aβ, limiting in vivo relevance.
  • Studying endogenous Aβ from human brain tissue is crucial but challenging.

Purpose of the Study:

  • To establish novel methods for analyzing distinct amyloid beta-protein (Aβ) species directly from human cortical tissue.
  • To develop a bioassay for assessing the neurotoxic impact of Aβ on human neurons.
  • To create a comprehensive platform for investigating Aβ activity and identifying neuroprotective strategies.

Main Methods:

  • Developed three distinct protocols to isolate and study different Aβ fractions: diffusible, solubilized, and true dimers.
  • Utilized induced pluripotent stem cell (iPSC)-derived human neurons for a neuritic integrity bioassay.
  • Combined extraction methods with the bioassay to analyze Aβ species and their effects.

Main Results:

  • Successfully isolated and characterized distinct Aβ species from human brain tissue.
  • Identified disease-related activity specifically in isolated Aβ dimers.
  • Demonstrated the neurotoxic effects of certain Aβ species on human neurons via the bioassay.

Conclusions:

  • The developed methods provide a robust platform for studying endogenous amyloid beta-protein (Aβ) in Alzheimer's disease (AD) research.
  • This approach allows for the investigation of specific Aβ species, particularly disease-relevant dimers.
  • The findings facilitate the identification of therapeutic targets and strategies to counteract Aβ-mediated neurotoxicity.