Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward More Translational Tumor Models: Breast dECM-Based 3D Systems Capture Native Microenvironmental Cues.

Bioengineering (Basel, Switzerland)·2026
Same author

A 3D-printed microdevice for dielectrophoretic torque-driven rotation of dielectric microspheres to support development of manipulation systems for zebrafish eggs.

Journal of micromechanics and microengineering : structures, devices, and systems·2026
Same author

Photoinduced electron transfer (PET) in ethaline-like solvents.

Physical chemistry chemical physics : PCCP·2026
Same author

Interdisciplinary Tools to Safeguard and Amplify Aquatic Genetic Resource Use: A Foundation for Industrial-Scale Quality Control for Fertilization.

Animals : an open access journal from MDPI·2026
Same author

Evaluation of Metaplastic Triple-Negative Breast Cancer Extracellular Matrix Structure and Protein Composition.

Bioengineering (Basel, Switzerland)·2026
Same author

Prevalence and Correlates of Nonsuicidal Self-Injury Among Transgender People: Results from a U.S. Probability Sample.

Psychology of sexual orientation and gender diversity·2025

Related Experiment Video

Updated: Aug 10, 2025

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.0K

A 3D Printed Vitrification Device for Storage in Cryopreservation Vials.

Yue Liu1,2, Andy Lin1, Terrence R Tiersch2

  • 1Department of Biological and Agricultural Engineering, Louisiana State University Agricultural Center, Louisiana State University, Baton Rouge, LA 70803, USA.

Applied Sciences (Basel, Switzerland)
|February 13, 2023
PubMed
Summary

Researchers developed a 3D printed Vitrification Device for Cryo-Vials (VDCV) to improve sperm cryopreservation in zebrafish. This low-cost, customizable device enhances sample handling, vitrification success, and storage efficiency for aquatic research.

Keywords:
3D printingdeviceopen hardwaresperm cryopreservationstandardizationvitrification

More Related Videos

Bulk Droplet Vitrification for Primary Hepatocyte Preservation
11:07

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Published on: October 25, 2019

5.7K
Author Spotlight: Advancing Fertility Preservation in Young Female Cancer Patients Through Ovarian Tissue Vitrification and In Vitro Follicular Growth
05:53

Author Spotlight: Advancing Fertility Preservation in Young Female Cancer Patients Through Ovarian Tissue Vitrification and In Vitro Follicular Growth

Published on: August 9, 2024

1.4K

Related Experiment Videos

Last Updated: Aug 10, 2025

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.0K
Bulk Droplet Vitrification for Primary Hepatocyte Preservation
11:07

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Published on: October 25, 2019

5.7K
Author Spotlight: Advancing Fertility Preservation in Young Female Cancer Patients Through Ovarian Tissue Vitrification and In Vitro Follicular Growth
05:53

Author Spotlight: Advancing Fertility Preservation in Young Female Cancer Patients Through Ovarian Tissue Vitrification and In Vitro Follicular Growth

Published on: August 9, 2024

1.4K

Area of Science:

  • Aquatic biology and cryopreservation research.
  • Development of specialized laboratory tools using additive manufacturing.

Background:

  • Sperm cryopreservation using vitrification is crucial for small-bodied aquatic animals like zebrafish (Danio rerio).
  • Existing vitrification tools are often adapted from human applications, presenting challenges for sperm cryopreservation in aquatic research.
  • Three-dimensional (3D) printing offers a novel approach to create customized and accessible cryopreservation tools.

Purpose of the Study:

  • To develop a 3D printed Vitrification Device for Cryo-Vials (VDCV) specifically for sperm cryopreservation.
  • To integrate the VDCV with existing vial storage systems for enhanced functionality.
  • To optimize the VDCV design for efficient sample handling, vitrification, storage, and identification.

Main Methods:

  • Designed and fabricated 36 configurations of the VDCV prototype, varying loop length, loop width, and support structures.
  • Investigated device handling orientations (horizontal and vertical plunging) during the vitrification process.
  • Utilized computer simulations to estimate sample cooling rates and experimentally assessed film retention and vitrification occurrence.

Main Results:

  • Computer simulations indicated rapid cooling rates (0.6-1.5 × 10^5 °C/min) across all configurations.
  • Axial support structures and horizontal plunging angles yielded higher film retention (≥92%) and vitrification success rates.
  • An 8 mm loop length demonstrated the highest overall vitrification occurrence (86-100%), with a capacity of approximately 6 × 10^7 sperm/vial.

Conclusions:

  • The 3D printed VDCV is a low-cost (<$0.5/unit), customizable, and effective tool for zebrafish sperm vitrification.
  • The VDCV facilitates improved sample handling, standardization, and storage, with potential for pooling sperm from multiple males.
  • Open-fabrication file sharing enables community access and fabrication using consumer-level 3D printers, promoting standardization in aquatic research.