Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

1.9K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
1.9K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

714
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
714
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

608
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
608
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

2.9K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
2.9K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

966
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
966
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

1.1K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autoantibodies Drive Fcγ Receptor-Dependent Colon Inflammation During Immune Checkpoint Blockade.

bioRxiv : the preprint server for biology·2026
Same author

Leveraging cDC1 biology and function for enhanced immunotherapy.

The Journal of experimental medicine·2026
Same author

Spatiotemporal dynamics of adoptively transferred stem-like CD8<sup>+</sup> T cells in the tumor microenvironment following vaccination.

bioRxiv : the preprint server for biology·2026
Same author

Reprogramming T cell-myeloid crosstalk overcomes immune resistance in colorectal cancer.

Cell reports. Medicine·2026
Same author

Mechanisms and Therapeutic Strategies to Overcome Immune Checkpoint Inhibitor Resistance in Melanoma, Head and Neck, and Triple-Negative Breast Cancers.

Journal of cellular immunology·2026
Same author

Improving peptide vaccine manufacturability without sacrificing immunogenicity: substitution of methionine and cysteine with oxidation-resistant isosteres.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Aug 10, 2025

Examination of Thymic Positive and Negative Selection by Flow Cytometry
14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

22.0K

Structural Basis for Self-Discrimination by Neoantigen-Specific TCRs.

John P Finnigan1,2,3,4, Jenna H Newman1,2,3, Yury Patskovsky5,6

  • 1Icahn School of Medicine at Mount Sinai; One Gustave L. Levy Pl., New York, NY.

Research Square
|February 13, 2023
PubMed
Summary

This study reveals shared structural features between tumor neoantigens and viral peptides, explaining their similar immunogenicity. Understanding these T cell receptor interactions is key for developing effective cancer immunotherapies.

Keywords:
TCRcancerimmunologyneoantigenstructure

More Related Videos

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

8.6K

Related Experiment Videos

Last Updated: Aug 10, 2025

Examination of Thymic Positive and Negative Selection by Flow Cytometry
14:29

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

22.0K
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.1K
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

8.6K

Area of Science:

  • Immunology
  • Structural Biology
  • Cancer Research

Background:

  • T cell receptor (TCR) interactions with tumor neoantigens (neoAg) presented by MHC class-I (MHC-I) are crucial for tumor cell lysis.
  • While adoptive transfer of neoAg-reactive T cells shows promise in cancer immunotherapy, the precise mechanisms of neoAg recognition are not fully understood.

Approach:

  • Identified specific neoAg:TCR pairs from the B16F10 melanoma model.
  • Determined high-resolution crystal structures of peptide-MHC (pMHC) and pMHC:TCR complexes.
  • Analyzed structural features enabling TCR recognition of neoAg.

Key Points:

  • A high-affinity TCR targeting H2-Db-restricted Hsf2K72N neoantigen was identified, demonstrating specific recognition of B16F10 melanoma cells.
  • Structural analysis revealed a unique hydrophobic arch in H2-Db facilitating extensive pMHC:TCR interactions.
  • The identified structural motifs in neoantigens resemble those found in viral peptides, suggesting a shared basis for immunogenicity.

Conclusions:

  • The structural stability and interaction patterns of neoAg:TCR complexes provide insights into T cell-mediated tumor surveillance.
  • Shared structural features between neoantigens and viral peptides may explain their comparable immunogenicity and inform the design of novel cancer immunotherapies.