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Fluorescence Lifetime Macro Imager for Biomedical Applications
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Light sheet autofluorescence lifetime imaging with a single photon avalanche diode array.

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This study introduces a novel light sheet imaging method for faster, label-free cellular metabolism analysis using fluorescence lifetime imaging microscopy (FLIM). The new system enables real-time monitoring of cellular functions in living organisms with enhanced speed and clarity.

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Area of Science:

  • Biophotonics and Imaging Science
  • Cellular Metabolism and Dynamics
  • Microscopy Techniques

Background:

  • Widefield fluorescence lifetime imaging microscopy (FLIM) offers faster imaging than laser scanning but struggles with label-free applications.
  • Epi-fluorescence geometry in FLIM is limited by out-of-focus light, hindering the detection of weak autofluorescence like NAD(P)H.
  • Accurate lifetime measurements are crucial for understanding cellular metabolic states.

Conclusions:

  • The developed light sheet FLIM system enables rapid, label-free imaging of cellular metabolism.
  • This technology significantly enhances 3D NAD(P)H FLIM for live cell and *in vivo* applications.
  • Offers a powerful tool for studying cellular metabolism and immune cell dynamics in real-time.