Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

261
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
261
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

262
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
262
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

833
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
833
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

499
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
499
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

486
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
486
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

3.4K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectroscopic discrimination of bacterial species of variable pathogenicity through explainable machine learning.

Nanoscale·2026
Same author

Critical interplay of defect engineering and plasmonics in hybrid nanostructures for ultrasensitive photo-enhanced Raman spectroscopy.

Nanoscale·2026
Same author

Genomic Surveillance of SARS-CoV-2 Variants Circulating in Rajasthan in 2025.

Cureus·2026
Same author

Comment on "Impact of pre-operative frailty on hospital length of stay in major abdominal oncological surgeries: A prospective, observational study".

Indian journal of anaesthesia·2026
Same author

From human to posthuman bias: mapping discrimination in futuristic societies through speculative fiction.

Frontiers in sociology·2026
Same author

Comparison of efficacy of ultrasound-guided single-level thoracic paravertebral block and complete antethoracic block for postoperative analgesia in modified radical mastectomy: A noninferiority randomized controlled trial.

Journal of anaesthesiology, clinical pharmacology·2026

Related Experiment Video

Updated: Aug 10, 2025

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

6.9K

Probing plasmon-induced surface reactions using two-dimensional correlation vibrational spectroscopy.

Ruchi Singh1, Vikas Yadav1, Soumik Siddhanta1

  • 1Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India. soumik@iitd.ac.in.

Physical Chemistry Chemical Physics : PCCP
|February 13, 2023
PubMed
Summary
This summary is machine-generated.

Surface plasmon resonance drives catalytic reactions using hot electrons. Combining plasmon-enhanced Raman and 2D correlation spectroscopy reveals insights into solvent effects and analyte orientation for controlled light-driven reactions.

More Related Videos

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

2.8K
Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

9.5K

Related Experiment Videos

Last Updated: Aug 10, 2025

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

6.9K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

2.8K
Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

9.5K

Area of Science:

  • Surface chemistry and catalysis
  • Nanomaterials science
  • Spectroscopic analysis

Background:

  • Surface plasmon resonance (SPR) utilizes hot electrons for catalytic conversion, crucial in heterogeneous catalysis.
  • Understanding reaction dynamics, influenced by nanoparticle composition, time, and conditions, is vital.
  • Current methods limit understanding of solvent effects, analyte orientation, and surface coverage in SPR-driven reactions.

Purpose of the Study:

  • To investigate plasmon-driven catalytic conversion of 4-nitrothiophenol on plasmonic nanoparticles.
  • To gain deeper insights into surface reaction dynamics and mechanisms.
  • To elucidate the interplay of solvent effects, analyte orientation, and surface packing.

Main Methods:

  • Combined use of plasmon-enhanced Raman spectroscopy and Two-dimensional correlation spectroscopy (2DCOS).
  • Operando spectroscopic analysis of 4-nitrothiophenol conversion on plasmonic nanoparticles.
  • Utilizing 2DCOS to analyze solvent effects, analyte orientation, and surface packing.

Main Results:

  • The combined technique yielded previously unobservable results in surface catalysis.
  • 2DCOS provided critical insights into the interplay of solvent effects, analyte orientation, and surface packing.
  • Selective formation of 4,4-dimercaptoazobenzene (DMAB) or 4-aminothiophenol (4-ATP) was observed based on analyte phase (ordered vs. disordered).

Conclusions:

  • The combined spectroscopic approach offers a powerful tool for studying plasmon-driven surface catalysis.
  • Precise control over light-driven reactions is achievable by manipulating analyte arrangement on the surface.
  • This study bridges the understanding gap in factors influencing SPR-driven catalytic reactions.