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Updated: Aug 10, 2025
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Published on: April 4, 2018
Identification by whole genome sequencing of genes associated with delayed postoperative hemorrhage in Scottish
Michael H Court1, Jennifer N Kiser2, Holly L Neibergs2
1Program in Individualized Medicine (PrIMe), Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA.
Background:
Delayed postoperative hemorrhage (DEPOH) is an important health concern for Scottish deerhounds.
Hypothesis/Objectives:
Identify genes associated with DEPOH in Scottish deerhounds.
Animals:
Two hundred sixty-nine privately owned Scottish deerhounds.
Methods:
Retrospective case-control study. DEPOH cases and controls were identified through an owner health survey. Genome-wide association analysis was performed using whole genome sequences from 8 cases and 17 controls. All cases and controls were genotyped for selected variants.
Results:
Of 269 dogs, 10 met inclusion and exclusion criteria for DEPOH, while 62 controls had undergone similar surgical procedures without DEPOH. Genome-wide association analysis identified a single locus on chromosome 9 spanning 40 genes. One of these genes (SERPINF2 encoding alpha-2 antiplasmin) was directly linked to the pathophysiology of DEPOH. The entire cohort was genotyped for a missense SERPINF2 variant (c.605 C>T; p.A202V). Compared to dogs with the reference C/C genotype, the likelihood of DEPOH was significantly higher for dogs with the T/T genotype (odds ratio [OR] = 1235; 95% confidence interval [CI] = 23-6752; P = 0.0005) and with the C/T genotype (OR = 28; 95% CI = 1.4-542; P = 0.03).
Conclusions And Clinical Importance:
SERPINF2 is associated with DEPOH in Scottish deerhounds. Genetic testing might be able to identify dogs that are susceptible to DEPOH.
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