BACTERIAL CONTAMINATION AND METHODS OF DECONTAMINATION OF BASES COMPLETE REMOVABLE PROSTHESES DURING THE APPLICATION...

Area of Science:

• Microbiology
• Biomedical Engineering
• Dental Materials Science

Background:

• Complete removable plastic dentures (CRPD) harbor a persistent microbiota with both aerobic and anaerobic species.
• The microbial composition of CRPD changes during orthopedic treatment stages.
• Understanding and improving decontamination methods for CRPD acrylic bases is crucial.

Purpose of the Study:

• To determine the bacterial contamination of CRPD acrylic bases during clinical use.
• To investigate changes in CRPD microbiota under different decontamination protocols.

Main Methods:

• CRPD acrylic base smears were Gram-stained, microscopically examined, and cultured on various agar media.
• Microbial identification utilized the LAHEMA test system, with quantification by colony-forming units (CFUs).
• Two decontamination modes were tested: Mode "A" (Sideex solution, glutaraldehyde-based) and Mode "B" (0.2% chlorhexidine bigluconate solution).

Main Results:

• Mode "B" (chlorhexidine) was more effective, reducing the microbiota species composition by 72.0%.
• Mode "B" significantly decreased aerobes from 31 lgCFU/ml to 8.7 lgCFU/ml and anaerobes from 42.7 lgCFU/ml to 14.6 lgCFU/ml.
• Mode "A" (Sideex) showed a smaller effect on species composition but significantly reduced microbial load (p<0.05).

Conclusions:

• Chlorhexidine bigluconate (Mode "B") is a more effective decontamination agent for CRPD acrylic bases than the tested glutaraldehyde-based solution (Mode "A").
• Effective decontamination protocols are essential to manage microbial contamination on CRPD.
• Further research into optimizing CRPD decontamination is warranted.