Optical Coherence Tomography Angiography Analysis Toolbox: A Repeatable and Reproducible Software Tool for...

Area of Science:

• Ophthalmology
• Medical Imaging
• Biomedical Engineering

Background:

• Optical Coherence Tomography Angiography (OCTA) is crucial for retinal vasculature analysis.
• Standardization of OCTA metrics across devices is challenging.
• The OAT software aims to address this variability.

Purpose of the Study:

• To evaluate the repeatability and reproducibility of the OCTA Analysis Toolkit (OAT).
• To assess the OAT's performance in computing OCTA metrics across different imaging devices.
• To establish the OAT as a reliable tool for consistent OCTA data analysis.

Main Methods:

• Fourteen participants underwent imaging using three different OCTA devices.
• The OAT software calculated key metrics: foveal avascular zone, vessel index, vessel length index, and vessel diameter index.
• Repeatability and reproducibility were quantified using coefficient of variation and interclass correlation coefficient (ICC).

Main Results:

• The OAT exhibited perfect repeatability (0% coefficient of variation) due to its deterministic nature.
• Excellent-to-good ICC values (>0.6) were observed for foveal avascular zone analysis across all devices.
• Most vessel index metrics showed good-to-fair or excellent-to-good ICC values, with one exception.

Conclusions:

• The OCTA Analysis Toolkit (OAT) is a reliable tool for quantitative OCTA analysis.
• The OAT facilitates consistent comparison of OCTA data acquired from different instruments.
• This standardization can lead to more reliable clinical and research applications of OCTA.