Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Classification and Identification01:28

Methods of Classification and Identification

80
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
80
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

51
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
51

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A new recombinant avian metapneumovirus vaccine candidate provides complete protection against the novel variant infectious bursal disease virus in chickens.

Veterinary research·2026
Same author

Advancing stress biotechnology for robust sustainable bioproduction.

Current opinion in biotechnology·2026
Same author

Single-Point versus Multi-Point Mechanical Loading in Membrane Deformation: Insights from Molecular Dynamics Simulations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

A Radial-Linear π-Conjugated Polymer by Integrating Poly(Para-phenylene Vinylene) and Cycloparaphenylene for Enhanced Optoelectronic and Electrochemical Performance.

Angewandte Chemie (International ed. in English)·2026
Same author

The perioperative effect of ERAS protocol in patients with lung cancer: a randomized controlled study.

Journal of cardiothoracic surgery·2026
Same author

Boundary echo control of S0-mode lamb waves using gradient phononic crystals.

Ultrasonics·2026

Related Experiment Video

Updated: Aug 10, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

425

Development and application of DETECTR-based rapid detection for pathogenic Bacillusanthracis.

Jianhao Xu1, Xinru Bai1, Xianglilan Zhang1

  • 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences (AMMS), Beijing, 100071, China.

Analytica Chimica Acta
|February 13, 2023
PubMed
Summary

A new DNA detection method accurately identifies Bacillus anthracis (B. anthracis) in under 40 minutes. This rapid test is crucial for detecting anthrax and potential biothreats with high sensitivity and specificity.

Keywords:
Bacillus anthracisBiothreatsCRISPR/Cas12aDETECTRIsothermal detectionNucleic acid detection

More Related Videos

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.2K
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

13.6K

Related Experiment Videos

Last Updated: Aug 10, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

425
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.2K
'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

13.6K

Area of Science:

  • Microbiology and Infectious Diseases
  • Molecular Diagnostics
  • Biotechnology

Background:

  • Bacillus anthracis (B. anthracis) causes anthrax, a serious disease requiring rapid detection for public health and biosecurity.
  • Existing diagnostic methods may lack the speed, sensitivity, or ease of use needed for prompt identification of B. anthracis.
  • Development of advanced detection platforms is essential for addressing natural outbreaks and deliberate bioterrorism threats.

Purpose of the Study:

  • To develop and evaluate a rapid, sensitive, and specific DNA detection platform for Bacillus anthracis.
  • To assess the efficacy of the DNA endonuclease targeted CRISPR trans reporter (DETECTR) system combined with recombinase polymerase amplification (RPA).
  • To validate the method using simulated clinical samples and historical environmental samples.

Main Methods:

  • A DETECTR detection platform targeting three specific B. anthracis DNA sequences (chromosomal marker, pagA, capA) was designed.
  • Recombinase polymerase amplification (RPA) was integrated with the DETECTR system for rapid nucleic acid amplification.
  • The method was tested on simulated blood samples and environmental samples from a World War II site.

Main Results:

  • The developed DETECTR-RPA method achieved identification of B. anthracis DNA in under 40 minutes with near two-copy sensitivity.
  • The system demonstrated high sensitivity and specificity in detecting B. anthracis in simulated clinical blood samples.
  • Positive B. anthracis samples from a historical World War II site were successfully detected, confirming the method's real-world applicability.

Conclusions:

  • The DETECTR-RPA platform offers a rapid, sensitive, and specific method for identifying pathogenic B. anthracis.
  • This user-friendly detection system can be adapted for portable devices, facilitating field deployment.
  • The method presents a novel and reliable option for screening and diagnosing anthrax and potential biothreats.