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Amplifluor-Based SNP Genotyping

Manmode Darpan Mohanrao1, Senapathy Senthilvel1, Yarabapani Rushwanth Reddy1

  • 1ICAR-Indian Institute of Oilseeds Research, Hyderabad, India.

Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
PubMed

Keywords:
AmplifluorGenotypic assaysSingle nucleotide polymorphisms (SNPs)

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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