Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

57.6K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.6K
Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogen peroxide is a central ROS regulator in plant immunity.

Physiology and molecular biology of plants : an international journal of functional plant biology·2025
Same author

Treading Toward Anterolateral Skull Base by Unlocking the Frontotemporal Dural Fold Along with Extradural Clinoidectomy: Translation from A Cadaver to Clinical Scenario.

Neurology India·2021
Same author

Recurrence of Symptoms on Long-Term Follow-up Following Subtotal Resection of Ruptured Intraventricular Dermoid.

Neurology India·2021
Same author

Identification of Novel Cathepsin B Inhibitors with Implications in Alzheimer's Disease: Computational Refining and Biochemical Evaluation.

Cells·2021
Same author

Modulating α-Synuclein Liquid-Liquid Phase Separation.

Biochemistry·2021
Same author

An integrated computational pipeline for designing high-affinity nanobodies with expanded genetic codes.

Briefings in bioinformatics·2021

Related Experiment Video

Updated: Aug 10, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.3K

Molecular Beacon Probe (MBP)-Based Real-Time PCR.

Gopal Kumar Prajapati1,2, Ashutosh Kumar1,3, Aakanksha Wany1,3

  • 1Department of Bioengineering and Biotechnology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India.

Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
PubMed
Summary

Molecular beacon probe (MBP) assays offer sensitive detection of specific gene sequences. This study applies MBP for identifying motif sequences in differentially expressed gene promoters using real-time PCR.

Keywords:
Identification of sequenceMolecular beacon probeMotifReal-time PCR

More Related Videos

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

13.7K
Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB

Published on: August 6, 2011

43.6K

Related Experiment Videos

Last Updated: Aug 10, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.3K
Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
15:01

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

Published on: August 6, 2012

13.7K
Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
10:15

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB

Published on: August 6, 2011

43.6K

Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • Probe-based techniques like TaqMan and minor groove binder (MGB) assays enhance gene detection via real-time PCR.
  • Molecular beacon probe (MBP) assays are hydrolysis probes that emit fluorescence upon binding to specific DNA sequences.
  • These methods offer superior sensitivity for target gene detection and quantification compared to conventional PCR.

Purpose of the Study:

  • To describe the application of molecular beacon probe (MBP) assays.
  • To identify specific motif sequences within the promoters of differentially expressed genes.

Main Methods:

  • Utilized real-time polymerase chain reaction (PCR).
  • Employed molecular beacon probes (MBPs) for sequence identification.
  • Focused on analyzing promoter regions of differentially expressed genes.

Main Results:

  • Demonstrated the utility of MBP assays for sequence identification.
  • Successfully identified motif sequences in gene promoters.

Conclusions:

  • MBP assays are effective tools for identifying specific DNA sequences.
  • This technique is valuable for analyzing regulatory elements like promoters in gene expression studies.