Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K
Real Time RT-PCR02:57

Real Time RT-PCR

57.6K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.6K
Southern Blot02:57

Southern Blot

19.8K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
19.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

VarLOCK: sequencing-independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance.

Scientific reports·2023
Same author

Optimized Loop-Mediated Isothermal Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART).

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Author Correction: Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells.

Scientific reports·2021
Same author

Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells.

Scientific reports·2020
Same author

Full Dynamic Range Quantification using Loop-mediated Amplification (LAMP) by Combining Analysis of Amplification Timing and Variance between Replicates at Low Copy Number.

Scientific reports·2020
Same author

Lack of specificity associated with using molecular beacons in loop mediated amplification assays.

BMC biotechnology·2019

Related Experiment Video

Updated: Aug 10, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K

Molecular Beacons - Loop-Mediated Amplification (MB-LAMP).

Patrick Hardinge1

  • 1School of Biosciences, Cardiff University, Cardiff, Wales, UK. hardingep@cardiff.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
PubMed
Summary

Molecular beacons (MBs) enhance specificity in loop-mediated amplification (LAMP) assays, improving detection of genetic variations. This method offers a robust solution for applications like genetically modified (GM) plant genotyping.

Keywords:
DNAGenotypingIsothermal nucleic acid amplification technology (iNAAT)Locking nucleic acids (LNAs)Loop-mediated amplification (LAMP)Molecular beacons (MBs)Single-nucleotide polymorphisms (SNPs)

More Related Videos

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K
A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

16.7K

Related Experiment Videos

Last Updated: Aug 10, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K
A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

16.7K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Polymerase chain reaction (PCR) uses molecular beacons (MBs) for high specificity in detecting mutations and single-nucleotide polymorphisms (SNPs).
  • Loop-mediated amplification (LAMP) is an isothermal nucleic acid amplification technology offering rapid, single-temperature reactions, suitable for plant genotyping.
  • LAMP's specificity is limited, leading to problematic false positives, despite requiring more primers than PCR.

Purpose of the Study:

  • To describe design considerations for molecular beacons (MBs) in loop-mediated amplification (LAMP) assays.
  • To present a method for MB-LAMP amplification and detection.
  • To demonstrate the application of MB-LAMP for detecting gene sequences in genetically modified (GM) maize.

Main Methods:

  • Adaptation of molecular beacons (MBs) for loop-mediated amplification (LAMP) by targeting single-stranded loop sequences.
  • Optimization of reaction conditions for MB-LAMP assays.
  • Application of MB-LAMP for gene sequence detection in genetically modified (GM) maize samples.

Main Results:

  • MBs significantly enhance the specificity of LAMP assays.
  • The developed MB-LAMP method enables sensitive and specific detection of target gene sequences.
  • Successful application demonstrated for identifying gene sequences in GM maize.

Conclusions:

  • Molecular beacons (MBs) are effective in improving the specificity of loop-mediated amplification (LAMP).
  • MB-LAMP provides a valuable tool for accurate genetic analysis, particularly in plant genotyping and GM detection.
  • This approach addresses the limitations of LAMP specificity, reducing false positives in molecular diagnostics.