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Tubulin-Based Polymorphism (TBP) in Plant Genotyping
Luca Braglia1, Floriana Gavazzi1, Silvia Gianì1
1Institute of Agricultural Biology and Biotechnology (IBBA), Milan, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
Abstract:
Tubulin-based polymorphism (TBP) is an intron length polymorphism (ILP) method widely applicable to any plant species and particularly suitable for a first and rapid classification of any plant genome. It is based on the selective, polymerase chain reaction (PCR)-based amplification of the two introns present at conserved positions within the coding sequences of plant β-tubulin genes. Amplification releases a simple yet distinctive genomic profile.

