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Updated: Aug 10, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Detection of DNA Modification Using Nanopore Sequencers.

Yoshikazu Furuta¹

¹Toyota Central R&D Labs., Inc., Nagakute, Japan. Yoshikazu.Furuta.ft@mosk.tytlabs.co.jp.

Methods in Molecular Biology (Clifton, N.J.)

|February 13, 2023

Summary

This summary is machine-generated.

Detecting DNA modifications is key for understanding gene regulation. This review covers nanopore sequencing methods, including a new protocol using MinION and Megalodon for precise DNA base mapping.

Keywords:

Basecalling DNA modification Epigenetics

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Last Updated: Aug 10, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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Published on: June 3, 2019

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Published on: December 14, 2017

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

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Published on: March 11, 2020

Area of Science:

Epigenetics and Genomics
Molecular Biology

Background:

DNA modification plays a critical role in epigenetic regulation, influencing gene expression and phenotype.
Accurate mapping of modified DNA bases is essential for a comprehensive understanding of these epigenetic mechanisms.
Traditional methods like bisulfite sequencing and PacBio SMRT sequencing exist, but nanopore sequencing offers an alternative detection approach.

Purpose of the Study:

To review existing nanopore sequencing-based methods for detecting DNA modifications.
To introduce a specific protocol for DNA modification detection utilizing the MinION and Megalodon platforms.

Main Methods:

Review of three distinct DNA modification detection strategies employing nanopore sequencing technology.
Detailed introduction of a protocol involving the MinION device and Megalodon software for base modification analysis.

Main Results:

Nanopore sequencing provides viable alternatives for detecting DNA modifications.
The presented MinION and Megalodon protocol offers a practical approach for precise DNA base mapping.

Conclusions:

Nanopore sequencing is a powerful tool for epigenetics research, complementing existing methods.
The MinION/Megalodon protocol facilitates accurate DNA modification detection, advancing gene regulation studies.