Preparation of Doxorubicin Liposomes by Remote Loading Method

Doxorubicin liposome is one of the most important nano-drug formulations. DOXIL, the first FDA-approved doxorubicin liposomes, is also the first nano-drug product in market. Since it was approved in 1995, DOXIL have been widely used in the treatment of various tumors. Several important technologies used in the development of doxorubicin liposomes, especially the remote loading technology, have an extremely important impact on the later liposome research and development. This article describes a protocol to prepare doxorubicin liposomes by remote loading in a laboratory.