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Establishment of an overactive bladder model in mice
Tengfei Lv1,2, Shan Zhong3, Xiao Guo4
1The Fourth School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
BMC Urology
|February 14, 2023
Summary
A partial bladder outlet obstruction (pBOO) successfully created an overactive bladder (OAB) mouse model. This model demonstrated increased urination frequency and bladder wall thickening, validating its use for OAB research.
Area of Science:
- Urology
- Experimental Medicine
- Animal Models
Background:
- Overactive bladder (OAB) is a common condition characterized by urinary urgency, frequency, and incontinence.
- Understanding the pathophysiology of OAB is crucial for developing effective treatments.
Purpose of the Study:
- To establish a reliable mouse model of overactive bladder (OAB) using partial bladder outlet obstruction (pBOO).
- To investigate the physiological and histological changes associated with pBOO in mice.
Main Methods:
- A partial bladder outlet obstruction (pBOO) model was created in female ICR mice by ligating the bladder outlet.
- Voiding spot on paper (VSOP), isolated detrusor muscle analysis, and HE staining were employed.
- Measurements included urination frequency, urine volume, bladder weight, bladder dimensions, and detrusor muscle thickness.
Main Results:
- The pBOO model exhibited significantly increased urination frequency and 12-hour urine volume compared to controls.
- Bladders in the pBOO group were significantly heavier, longer, and had thicker walls.
- Detrusor muscle strips from pBOO mice showed increased sensitivity to carbachol stimulation.
Conclusions:
- The partial bladder outlet obstruction (pBOO) method effectively established a mouse model for overactive bladder (OAB).
- This validated model provides a platform for further research into OAB mechanisms and therapeutic interventions.
