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[Selective media for Pasteurella pneumotropica].

K Mikazuki1, K Takahashi, K Nanba

  • 1Aburahi Laboratories, Shionogi and Co., Ltd., Shiga, Japan.

Jikken Dobutsu. Experimental Animals
|July 1, 1987
PubMed
Summary

Researchers identified Pasteurella pneumotropica in DS mouse abscesses. Novel culture media (NKBT and TGN broth) enable selective isolation and detection of latent infections without animal sacrifice.

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Area of Science:

  • Microbiology
  • Veterinary Pathology

Context:

  • Subcutaneous abscesses were observed in a laboratory colony of DS mice.
  • Abscesses primarily affected multiparous female mice over 100 days old, while virgin females remained unaffected.

Purpose:

  • To identify the causative agent of subcutaneous abscesses in DS mice.
  • To develop selective culture media for Pasteurella pneumotropica isolation.
  • To establish a method for detecting latent infections without sacrificing animals.

Summary:

  • Biological examinations identified Pasteurella pneumotropica as the causative agent.
  • Minimum inhibitory concentration (MIC) studies revealed potassium tellurite, kanamycin, and bacitracin as effective selective agents.
  • A novel NKBT medium (heart infusion agar with Fildes digested blood and selective agents) and TGN broth were developed.
  • Direct swabbing sufficed for pharyngeal isolation, while pre-multiplication in TGN broth significantly improved gut and fecal sample recovery.

Impact:

  • The developed culture methods allow for easy detection of latent Pasteurella pneumotropica infections.
  • This facilitates non-invasive monitoring of bacterial infections in laboratory mouse colonies.
  • Improved diagnostic techniques contribute to better animal health management and research integrity.

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